Anti-Leishmania amazonensis activity of Serjania lethalis A. St.-Hil

Abstract

Extracts of Serjania lethalis A. St.-Hil leaves and stems were tested in order to identify potential agents against Leishmania amazonensis. The hexane fraction (HF) and dichloromethane subfractions (DDF and MDF) showed leishmanicidal effect. The anti-promastigote IC₅₀ values were 10.29 (HF), 11.41 (DDF) and 28.33μg⁄mL (MDF); whereas those against amastigote were 7.2 (HF), 8.1 (DDF) and 6.5μg⁄mL (MDF). Among the fractions and subfractions assayed, only HF altered the cell cycle of the parasite, increasing 3-fold the number of cells in the sub-G0/G1 phase. HF also changed the parasite mitochondrial membrane potential (ΔΨm) and the percentage of annexin-V-propidium iodide positive promastigotes. Our evaluations of the IC₅₀ values showed that HF, DDF and MDF decreased NO production in infected macrophages stimulated with IFN-γ and LPS. Moreover, HF increased the production of TNF-α in Leishmania infected macrophages. This paper reports for the first time the leishmanicidal activity of extracts and fractions of Serjania lethalis leaves and also characterizes its leishmanicidal and immunomodulatory properties.

Keywords: Hexane leaves extract; Intracellular amastigotes; Leishmania; Leishmanicidal activity; Promastigotes; Serjania.