Abstract
In this study, we attempted to explore the effect and possible mechanism of Andrographolide on OVA-induced asthma. OVA challenge induced significant airway inflammatory cell recruitment and lung histological alterations, which were ameliorated by Andrographolide. The protein levels of cytokines in bron-choalveolar fluid (BALF) and serum were reduced by Andrographolide administration as well as the mRNA levels in lung tissue. Mechanically, Andrographolide markedly hampered the activation of nuclear factor-κB (NF-κB) and NLRP3 inflammasome both in vivo and vitro thus decreased levels of TNF-α and IL-1β. Finally, we confirmed that ROS scavenging was responsible for Andrographolide's inactivation of NF-κB and NLRP3 inflammasome signaling. Our study here revealed the effect and possible mechanism of Andrographolide on asthma, which may represent a new therapeutic approach for treating this disease.
Keywords:
Immune response; Immunity; Immunology and Microbiology Section; NF-κB; NLRP3; ROS; andrographolide; asthma.
MeSH terms
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Animals
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Anti-Asthmatic Agents / pharmacology*
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Anti-Inflammatory Agents / pharmacology*
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Antioxidants / pharmacology*
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Asthma / chemically induced
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Asthma / metabolism
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Asthma / pathology
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Asthma / prevention & control*
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Bronchoalveolar Lavage Fluid / chemistry
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Cytokines / genetics
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Cytokines / metabolism
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Disease Models, Animal
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Diterpenes / pharmacology*
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Dose-Response Relationship, Drug
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Female
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Gene Expression Regulation
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Inflammasomes / drug effects*
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Inflammasomes / metabolism
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Lung / drug effects*
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Lung / metabolism
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Lung / pathology
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Mice, Inbred C57BL
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NF-kappa B / metabolism*
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NLR Family, Pyrin Domain-Containing 3 Protein / metabolism*
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Ovalbumin*
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Oxidative Stress / drug effects*
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Pneumonia / chemically induced
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Pneumonia / metabolism
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Pneumonia / pathology
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Pneumonia / prevention & control*
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Reactive Oxygen Species / metabolism*
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Signal Transduction / drug effects
Substances
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Anti-Asthmatic Agents
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Anti-Inflammatory Agents
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Antioxidants
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Cytokines
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Diterpenes
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Inflammasomes
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NF-kappa B
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NLR Family, Pyrin Domain-Containing 3 Protein
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Nlrp3 protein, mouse
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Reactive Oxygen Species
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andrographolide
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Ovalbumin