Abstract
Phosphoinositide-phospholipase C-β1 (PLC-β1) plays a crucial role in the initiation of the genetic program responsible for muscle differentiation and osteogenesis. During myogenic differentiation of murine C2C12 myoblasts, PLC-β1 signaling pathway involves the Inositol Polyphosphate Multikinase (IPMK) and β-catenin as downstream effectors. By means of c-jun binding to cyclin D3 promoter, the activation of PLC-β1 pathway determines cyclin D3 accumulation. However, osteogenesis requires PLC-β1 expression and up-regulation but it does not affect cyclin D3 levels, suggesting that the two processes require the activation of different mediators.
Copyright © 2016 Elsevier Ltd. All rights reserved.
MeSH terms
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Animals
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Cell Differentiation
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Cell Line
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Cyclin D3 / genetics
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Cyclin D3 / metabolism
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Gene Expression Regulation, Developmental
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JNK Mitogen-Activated Protein Kinases / genetics
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JNK Mitogen-Activated Protein Kinases / metabolism
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Mice
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Muscle Development / genetics*
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Myoblasts / cytology
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Myoblasts / metabolism*
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Osteoblasts / cytology
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Osteoblasts / metabolism*
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Osteogenesis / genetics*
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Phospholipase C beta / genetics*
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Phospholipase C beta / metabolism
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Phosphotransferases (Alcohol Group Acceptor) / genetics
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Phosphotransferases (Alcohol Group Acceptor) / metabolism
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Promoter Regions, Genetic
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Protein Binding
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Signal Transduction
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beta Catenin / genetics
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beta Catenin / metabolism
Substances
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CTNNB1 protein, mouse
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Ccnd3 protein, mouse
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Cyclin D3
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beta Catenin
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Phosphotransferases (Alcohol Group Acceptor)
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inositol polyphosphate multikinase
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JNK Mitogen-Activated Protein Kinases
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Phospholipase C beta
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Plcb1 protein, mouse