Molecular cloning of orange-spotted grouper (Epinephelus coioides) heat shock transcription factor 1 isoforms and characterization of their expressions in response to nodavirus

Ting-Yu Wang; Young-Mao Chen; Tzong-Yueh Chen

doi:10.1016/j.fsi.2016.10.032

Molecular cloning of orange-spotted grouper (Epinephelus coioides) heat shock transcription factor 1 isoforms and characterization of their expressions in response to nodavirus

Fish Shellfish Immunol. 2016 Dec:59:123-136. doi: 10.1016/j.fsi.2016.10.032. Epub 2016 Oct 20.

Authors

Ting-Yu Wang¹, Young-Mao Chen², Tzong-Yueh Chen³

Affiliations

¹ Laboratory of Molecular Genetics, Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 70101, Taiwan; Translational Center for Marine Biotechnology, National Cheng Kung University, Tainan 70101, Taiwan.
² Laboratory of Molecular Genetics, Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 70101, Taiwan; Department of Biotechnology and Bioindustry Sciences, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 70101, Taiwan; Translational Center for Marine Biotechnology, National Cheng Kung University, Tainan 70101, Taiwan; Agriculture Biotechnology Research Center, National Cheng Kung University, Tainan 70101, Taiwan.
³ Laboratory of Molecular Genetics, Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 70101, Taiwan; Department of Biotechnology and Bioindustry Sciences, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 70101, Taiwan; Translational Center for Marine Biotechnology, National Cheng Kung University, Tainan 70101, Taiwan; Agriculture Biotechnology Research Center, National Cheng Kung University, Tainan 70101, Taiwan; University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan 70101, Taiwan. Electronic address: ibcty@mail.ncku.edu.tw.

PMID: 27773730
DOI: 10.1016/j.fsi.2016.10.032

Abstract

Heat shock transcription factor 1 (HSF1) regulates heat shock proteins (HSPs), which assist in protein folding and inhibit protein denaturation following stress. HSF1 was firstly cloned from orange-spotted grouper and exists as two isoforms, one with (osgHSF1a) and one without (osgHSF1b) exon 11. Heat exposure increased the expression of osgHSF1b while cold exposure increased that of osgHSF1a. Both isoforms were mainly expressed in the brains, eyes, and fins. Expression of osgHSF1b was higher than osgHSF1a during development. Poly I:C and LPS could also induce osgHSF1 isoforms expression differentially. Exposure to nervous necrosis virus (NNV) increased the level of both osgHSF1 isoforms at 12 h. GF-1 cells with overexpression of osgHSF1 isoforms enhanced viral loads within 24 h, whereas both pharmacological inhibition and RNA interference of HSF1 reduced virus infection. This study shows that osgHSF1 can support the early stage of virus infection and provides a new insight into the molecular regulation of osgHSF1 between the influence of temperatures and immunity.

Keywords: Heat shock protein; Heat shock transcription factor; Nervous necrosis virus; Orange-spotted grouper.

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Bass*
Cloning, Molecular
DNA, Complementary / genetics
DNA, Complementary / metabolism
DNA-Binding Proteins / chemistry
DNA-Binding Proteins / genetics*
DNA-Binding Proteins / metabolism
Fish Diseases / immunology*
Fish Proteins / chemistry
Fish Proteins / genetics*
Fish Proteins / metabolism
Gene Expression Regulation / immunology*
Heat Shock Transcription Factors
Hot Temperature / adverse effects
Immunity / drug effects
Lipopolysaccharides / pharmacology
Nodaviridae / physiology
Phylogeny
Poly I-C / pharmacology
Protein Isoforms / chemistry
Protein Isoforms / genetics
Protein Isoforms / metabolism
RNA Virus Infections / immunology
RNA Virus Infections / veterinary*
Sequence Alignment / veterinary
Transcription Factors / chemistry
Transcription Factors / genetics*
Transcription Factors / metabolism

Substances

DNA, Complementary
DNA-Binding Proteins
Fish Proteins
Heat Shock Transcription Factors
Lipopolysaccharides
Protein Isoforms
Transcription Factors
Poly I-C