Integrated mRNA and miRNA transcriptome reveal a cross-talk between developing response and hormone signaling for the seed kernels of Siberian apricot

Sci Rep. 2016 Oct 20:6:35675. doi: 10.1038/srep35675.

Abstract

Recently, our transcriptomic analysis has identified some functional genes responsible for oil biosynthesis in developing SASK, yet miRNA-mediated regulation for SASK development and oil accumulation is poorly understood. Here, 3 representative periods of 10, 30 and 60 DAF were selected for sRNA sequencing based on the dynamic patterns of growth tendency and oil content of developing SASK. By miRNA transcriptomic analysis, we characterized 296 known and 44 novel miRNAs in developing SASK, among which 36 known and 6 novel miRNAs respond specifically to developing SASK. Importantly, we performed an integrated analysis of mRNA and miRNA transcriptome as well as qRT-PCR detection to identify some key miRNAs and their targets (miR156-SPL, miR160-ARF18, miR164-NAC1, miR171h-SCL6, miR172-AP2, miR395-AUX22B, miR530-P2C37, miR393h-TIR1/AFB2 and psi-miRn5-SnRK2A) potentially involved in developing response and hormone signaling of SASK. Our results provide new insights into the important regulatory function of cross-talk between development response and hormone signaling for SASK oil accumulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Expression Profiling*
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Plant
  • MicroRNAs / analysis*
  • Oils, Volatile / metabolism
  • Plant Development
  • Plant Growth Regulators / metabolism*
  • Prunus armeniaca / growth & development*
  • RNA, Messenger / analysis*
  • Real-Time Polymerase Chain Reaction
  • Seeds / genetics
  • Seeds / growth & development*
  • Seeds / metabolism
  • Sequence Analysis, DNA
  • Signal Transduction*
  • Time Factors

Substances

  • MicroRNAs
  • Oils, Volatile
  • Plant Growth Regulators
  • RNA, Messenger