Method for Analysis of Matrix Degradation by CCN2 Through the MMP/TIMP System

Susan V McLennan; Danqing Min; Xiaoyu Wang; Stephen M Twigg

doi:10.1007/978-1-4939-6430-7_44

Method for Analysis of Matrix Degradation by CCN2 Through the MMP/TIMP System

Methods Mol Biol. 2017:1489:523-532. doi: 10.1007/978-1-4939-6430-7_44.

Authors

Susan V McLennan^{1

2}, Danqing Min^{1

2}, Xiaoyu Wang¹, Stephen M Twigg^{3

4}

Affiliations

¹ Greg Brown Diabetes and Endocrinology Research Laboratory, Sydney Medical School, Charles Perkins Centre, The University of Sydney, Sydney, NSW, 2006, Australia.
² Department of Endocrinology, Royal Prince Alfred Hospital, Camperdown, NSW, 2050, Australia.
³ Greg Brown Diabetes and Endocrinology Research Laboratory, Sydney Medical School, Charles Perkins Centre, The University of Sydney, Sydney, NSW, 2006, Australia. stephen.twigg@sydney.edu.au.
⁴ Department of Endocrinology, Royal Prince Alfred Hospital, Camperdown, NSW, 2050, Australia. stephen.twigg@sydney.edu.au.

PMID: 27734403
DOI: 10.1007/978-1-4939-6430-7_44

Abstract

Many studies have shown effects of members of the CCN family on matrix synthesis and accumulation but few have examined degradative pathways. This scarcity of information is in part due to the lack of suitable model systems. Here we describe methods for making rhCCN2 and also for the preparation of a biosynthetically labeled matrix substrate that can be used to measure the effect of CCN on cellular or secreted degradative pathways.

Keywords: CCN2; Connective tissue growth factor; Matrix degradation pathways; Matrix metalloproteinase; Tissue inhibitor of matrix metalloproteinase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Affinity
Connective Tissue Growth Factor / genetics
Connective Tissue Growth Factor / isolation & purification
Connective Tissue Growth Factor / metabolism*
Extracellular Matrix / metabolism*
Humans
Matrix Metalloproteinases / genetics
Matrix Metalloproteinases / metabolism*
Proteolysis
Recombinant Proteins / isolation & purification
Recombinant Proteins / metabolism
Substrate Specificity
Tissue Inhibitor of Metalloproteinases / genetics
Tissue Inhibitor of Metalloproteinases / metabolism*

Substances

Recombinant Proteins
Tissue Inhibitor of Metalloproteinases
Connective Tissue Growth Factor
Matrix Metalloproteinases