Analysis of Transcytosis of CCN2 by Chondrocytes

Kazumi Kawata; Satoshi Kubota; Masaharu Takigawa

doi:10.1007/978-1-4939-6430-7_33

Analysis of Transcytosis of CCN2 by Chondrocytes

Methods Mol Biol. 2017:1489:405-413. doi: 10.1007/978-1-4939-6430-7_33.

Authors

Kazumi Kawata¹, Satoshi Kubota^{2

3}, Masaharu Takigawa⁴

Affiliations

¹ Department of Biochemistry and Molecular Dentistry, Okayama University Dental School and Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.
² Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School/Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.
³ Department of MembraneBiochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
⁴ Advanced Research Center for Oraland Craniofacial Sciences, Okayama University Dental School/Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Kita-ku, Okayama, 700-8525, Japan. takigawa@md.okayama-u.ac.jp.

PMID: 27734392
DOI: 10.1007/978-1-4939-6430-7_33

Abstract

Transcytosis is a mechanism for the transcellular transport of biomolecules. Analysis of transcytosis is frequently performed in cells with distinct polarity, such as brain endothelial cells. However, in cells without evident polarity, analysis of transcytosis has not been performed. Here, we describe a method for analyzing transcytosis of a CCN family protein through chondrocytic cells having no apparent polarity.

Keywords: Analysis of transcytosis; Cells without polarity; Chondrocytes; FLAG-tagged recombinant CCN2 protein; Transwell.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blotting, Western
Cell Line
Chondrocytes / metabolism*
Connective Tissue Growth Factor / genetics*
Connective Tissue Growth Factor / metabolism*
Humans
Recombinant Fusion Proteins
Transcytosis / genetics*

Substances

Recombinant Fusion Proteins
Connective Tissue Growth Factor