Isolation of Nuclei and Nucleoli

Methods Mol Biol. 2017:1511:31-44. doi: 10.1007/978-1-4939-6533-5_3.

Abstract

Here we describe methods for producing nuclei from Arabidopsis suspension cultures or root tips of Arabidopsis, wheat, or pea. These methods could be adapted for other species and cell types. The resulting nuclei can be further purified for use in biochemical or proteomic studies, or can be used for microscopy. We also describe how the nuclei can be used to obtain a preparation of nucleoli.

Keywords: Immunofluorescence; Nuclear isolation; Nuclei; Nucleoli; Proteomics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / chemistry
  • Arabidopsis / chemistry*
  • Cell Fractionation / instrumentation
  • Cell Fractionation / methods*
  • Cell Nucleolus / chemistry*
  • Cell Nucleolus / ultrastructure
  • Cell Nucleus / chemistry*
  • Cell Nucleus / ultrastructure
  • Centrifugation, Density Gradient / instrumentation
  • Centrifugation, Density Gradient / methods
  • Culture Media / chemistry
  • Fluorescent Antibody Technique
  • Meristem / chemistry
  • Pisum sativum / chemistry*
  • Protoplasts / chemistry
  • Protoplasts / ultrastructure
  • Seeds / chemistry
  • Staining and Labeling / methods
  • Triticum / chemistry*

Substances

  • Antibodies
  • Culture Media