Molecular characterization of a novel endornavirus from the phytopathogenic fungus Botrytis cinerea

Arch Virol. 2017 Jan;162(1):313-316. doi: 10.1007/s00705-016-3106-2. Epub 2016 Oct 8.

Abstract

The complete sequence of a novel endornavirus (Botrytis cinerea endornavirus 1, BcEV1) from the phytopathogenic fungus Botrytis cinerea strain HBtom-372 was determined. The BcEV1 coding strand is 11,557 nucleotides long, possessing an open reading frame (ORF) that codes for a polyprotein of 3,787 amino acid residues and lacks a site-specific nick. The polyprotein contains viral methyltransferase (MTR) domain, a cysteine-rich region (CRR), two putative viral helicase (DEXDc-like and Hel-1) domains, and an RNA-dependent RNA polymerase_2 (RdRp_2) domain. In phylogenetic analysis, BcEV1 clustered with several fungal endornaviruses, forming an independent clade, and it was detected in 4.2 % of B. cinerea strains collected from central China.

MeSH terms

  • Botrytis / virology*
  • China
  • Cluster Analysis
  • Fungal Viruses / classification
  • Fungal Viruses / genetics*
  • Fungal Viruses / isolation & purification*
  • Genome, Viral*
  • Open Reading Frames
  • Phylogeny
  • Polyproteins / genetics
  • Protein Domains
  • RNA Viruses / classification
  • RNA Viruses / genetics*
  • RNA Viruses / isolation & purification*
  • RNA, Viral / genetics
  • Sequence Analysis, DNA*
  • Sequence Homology
  • Viral Proteins / genetics

Substances

  • Polyproteins
  • RNA, Viral
  • Viral Proteins