Tutorial on estimating the limit of detection using LC-MS analysis, part II: Practical aspects

Hanno Evard; Anneli Kruve; Ivo Leito

doi:10.1016/j.aca.2016.08.042

Tutorial on estimating the limit of detection using LC-MS analysis, part II: Practical aspects

Anal Chim Acta. 2016 Oct 26:942:40-49. doi: 10.1016/j.aca.2016.08.042. Epub 2016 Sep 6.

Authors

Hanno Evard¹, Anneli Kruve², Ivo Leito³

Affiliations

¹ University of Tartu, Institute of Chemistry, Ravila 14a, Tartu, 50411, Estonia. Electronic address: evardhanno@gmail.com.
² University of Tartu, Institute of Chemistry, Ravila 14a, Tartu, 50411, Estonia. Electronic address: anneli.kruve@ut.ee.
³ University of Tartu, Institute of Chemistry, Ravila 14a, Tartu, 50411, Estonia. Electronic address: ivo.leito@ut.ee.

PMID: 27720120
DOI: 10.1016/j.aca.2016.08.042

Abstract

In part II of this tutorial, the investigated approaches of estimating the limit of detection (LOD) are applied to experimental data from LC-MS measurements. Important practical aspects specific to LC-MS and related to LOD are reviewed. The results of different tests of estimating linearity and scedasticity are compared. LOD estimates obtained with different approaches (for both simple characterization of the analysis method and accurate interpretation of the results) are applied to the data and the obtained values are compared. As a conclusion, a decision tree is proposed for estimating LOD for analytical methods using the LC-MS technique.

Keywords: Decision limit; Detection capability; Detection limit; Limit of detection; Liquid chromatography; Mass spectrometry.

Publication types

Review