Direct real-time detection of single proteins using silicon nanowire-based electrical circuits

Jie Li; Gen He; Hiroshi Ueno; Chuancheng Jia; Hiroyuki Noji; Chuanmin Qi; Xuefeng Guo

doi:10.1039/c6nr04103e

Direct real-time detection of single proteins using silicon nanowire-based electrical circuits

Nanoscale. 2016 Sep 15;8(36):16172-16176. doi: 10.1039/c6nr04103e.

Authors

Jie Li¹, Gen He¹, Hiroshi Ueno², Chuancheng Jia³, Hiroyuki Noji², Chuanmin Qi⁴, Xuefeng Guo⁵

Affiliations

¹ Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, P. R. China. qichuanmin@bnu.edu.cn and Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China. guoxf@pku.edu.cn.
² Department of Applied Chemistry, School of Engineering, The University of Tokyo, Tokyo 113-8654, Japan. hnoji@appchem.t.u-tokyo.ac.jp.
³ Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China. guoxf@pku.edu.cn.
⁴ Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, P. R. China. qichuanmin@bnu.edu.cn.
⁵ Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China. guoxf@pku.edu.cn and Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, P. R. China.

PMID: 27714062
DOI: 10.1039/c6nr04103e

Abstract

We present an efficient strategy through surface functionalization to build a single silicon nanowire field-effect transistor-based biosensor that is capable of directly detecting protein adsorption/desorption at the single-event level. The step-wise signals in real-time detection of His-tag F₁-ATPases demonstrate a promising electrical biosensing approach with single-molecule sensitivity, thus opening up new opportunities for studying single-molecule biophysics in broad biological systems.

MeSH terms

Biosensing Techniques*
Nanowires*
Proteins / isolation & purification*
Proton-Translocating ATPases / isolation & purification
Silicon*
Transistors, Electronic

Substances

Proteins
Proton-Translocating ATPases
Silicon