Mesorhizobium olivaresii sp. nov. isolated from Lotus corniculatus nodules

María J Lorite; José David Flores-Félix; Álvaro Peix; Juan Sanjuán; Encarna Velázquez

doi:10.1016/j.syapm.2016.09.003

Mesorhizobium olivaresii sp. nov. isolated from Lotus corniculatus nodules

Syst Appl Microbiol. 2016 Dec;39(8):557-561. doi: 10.1016/j.syapm.2016.09.003. Epub 2016 Sep 24.

Authors

María J Lorite¹, José David Flores-Félix², Álvaro Peix³, Juan Sanjuán¹, Encarna Velázquez⁴

Affiliations

¹ Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidin, CSIC, Granada, Spain.
² Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
³ Unidad Asociada Universidad de Salamanca-CSIC "Interacciones Planta-Microorganismo", Spain; IRNASA-CSIC, Salamanca,Spain.
⁴ Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain; Unidad Asociada Universidad de Salamanca-CSIC "Interacciones Planta-Microorganismo", Spain. Electronic address: evp@usal.es.

PMID: 27712916
DOI: 10.1016/j.syapm.2016.09.003

Abstract

In this study four Mesorhizobium strains isolated from Lotus corniculatus nodules in Granada (Spain) were characterized. Their 16S rRNA gene sequences were closely related to those of M. albiziae LMG 23507^T and M. chacoense Pr5^T showing 99.4 and 99.2% similarity values, respectively. The analysis of concatenated rpoB, recA, atpD and glnII genes showed they formed a cluster with internal similarities higher than 97%. The closest species also were M. albiziae LMG 23507^T and M. chacoense Pr5^T showing similarity values lower than 92% in rpoB, recA and glnII genes and lower than 96.5% in the atpD gene. These results indicated that the L. corniculatus strains belong to a new species of genus Mesorhizobium which was confirmed by DNA-DNA hybridization and phenotypic characterization. Therefore a new species with the name Mesorhizobium olivaresii sp. nov. is proposed, and the type strain is CPS13^T (LMG 29295^T=CECT 9099^T).

Keywords: Lotus corniculatus; Mesorhizobium; Spain.

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacterial Typing Techniques
Carbohydrate Metabolism / physiology
DNA, Bacterial / genetics*
DNA-Directed RNA Polymerases / genetics
Genes, Essential / genetics*
Lotus / microbiology*
Mesorhizobium / classification*
Mesorhizobium / drug effects
Mesorhizobium / genetics*
Mesorhizobium / isolation & purification
Microbial Sensitivity Tests
Nucleic Acid Hybridization
RNA, Ribosomal, 16S / genetics
Rec A Recombinases / genetics
Root Nodules, Plant / microbiology*
Sequence Analysis, DNA
Spain
Transcription Factors / genetics

Substances

Anti-Bacterial Agents
DNA, Bacterial
RNA, Ribosomal, 16S
Transcription Factors
Rec A Recombinases
DNA-Directed RNA Polymerases