Nanohybride Materials Based on Magnetite-Gold Nanoparticles for Diagnostics of Prostate Cancer: Synthesis and In Vitro Testing

A E Machulkin; A S Garanina; O A Zhironkina; E K Beloglazkina; N V Zyk; A G Savchenko; V E Kotelyanskii; A G Mazhuga

doi:10.1007/s10517-016-3490-3

Nanohybride Materials Based on Magnetite-Gold Nanoparticles for Diagnostics of Prostate Cancer: Synthesis and In Vitro Testing

Bull Exp Biol Med. 2016 Sep;161(5):706-710. doi: 10.1007/s10517-016-3490-3. Epub 2016 Oct 4.

Authors

A E Machulkin^{1

2}, A S Garanina³, O A Zhironkina⁴, E K Beloglazkina^{5

3}, N V Zyk⁵, A G Savchenko³, V E Kotelyanskii⁵, A G Mazhuga^{5

3}

Affiliations

¹ Laboratory of Tissue-Specific Ligands, Faculty of Chemistry, M. V. Lomonosov Moscow State University, Moscow, Russia. alekseymachulkin@rambler.ru.
² Laboratory of Biomedical Nanomaterials, National University of Science and Technology, Moscow Institute of Steel and Alloys (NUST MISiS), Moscow, Russia. alekseymachulkin@rambler.ru.
³ Laboratory of Biomedical Nanomaterials, National University of Science and Technology, Moscow Institute of Steel and Alloys (NUST MISiS), Moscow, Russia.
⁴ Department of Electronic Microscopy, A. N. Belozersky Research Institute of Physical and Chemical Biology, Moscow, Russia.
⁵ Laboratory of Tissue-Specific Ligands, Faculty of Chemistry, M. V. Lomonosov Moscow State University, Moscow, Russia.

PMID: 27704352
DOI: 10.1007/s10517-016-3490-3

Abstract

We synthesized a fluorescence conjugate and modified magnetite-gold nanoparticles carrying prostate specific membrane antigen (PSMA) as the ligand. Analysis of their binding to human prostate cancer cell lines PC-3 (PSMA^-) and LNCaP (PSMA⁺) showed selective interaction of the synthesized conjugate and modified nanoparticles with LNCaP cells. These findings suggest that these nanoparticles can be used in tissue-specific magnetic-resonance imaging.

Keywords: diagnostics; magnetic-resonance imaging (MRI); nanoparticles; prostate cancer; prostate-specific membrane antigen.

MeSH terms

Cell Line, Tumor
Contrast Media / chemical synthesis*
Contrast Media / metabolism
Cytoplasm / metabolism
Gold / chemistry
Humans
Magnetite Nanoparticles / chemistry
Male
Nanoconjugates / chemistry
Prostatic Neoplasms / diagnostic imaging*

Substances

Contrast Media
Magnetite Nanoparticles
Nanoconjugates
Gold