Conversion of Thin Stillage Compounds Using Endemic Bacteria Augmented with Lactobacillus panis PM1B

Kornsulee Ratanapariyanuch; Youn Young Shim; Martin J T Reaney

doi:10.1021/acs.jafc.6b00643

Conversion of Thin Stillage Compounds Using Endemic Bacteria Augmented with Lactobacillus panis PM1B

J Agric Food Chem. 2016 Oct 26;64(42):7940-7948. doi: 10.1021/acs.jafc.6b00643. Epub 2016 Oct 18.

Authors

Kornsulee Ratanapariyanuch¹, Youn Young Shim^{1

2

3}, Martin J T Reaney^{1

2

3}

Affiliations

¹ Department of Plant Sciences, University of Saskatchewan , 51 Campus Drive, Saskatoon, Saskatchewan S7N 5A8, Canada.
² Prairie Tide Chemicals Inc. , 102 Melville Street, Saskatoon, Saskatchewan S7J 0R1, Canada.
³ Guangdong Saskatchewan Oilseed Joint Laboratory, Department of Food Science and Engineering, Jinan University , 601 Huangpu Avenue West, Guangzhou, Guangdong 510632, China.

PMID: 27696845
DOI: 10.1021/acs.jafc.6b00643

Abstract

A consortium of organisms, endemic in wheat-based thin stillage (W-TS), obtained from a commercial ethanol production converts glycerol to 1,3-propanediol (1,3-PD) and lactic acid to acetic acid. We sought to improve conditions for 1,3-PD and acetic acid production to be used in future studies of industrial isolation of these compounds from two-stage fermentation. Occasionally, stillage fermentation proceeded slowly, but an inoculum of Lactobacillus panis PM1B augmented both fermentation rate and extent. Fermentation rate and product yield were enhanced by adjusting pH to 5 daily and adding glucose and glycerol (molar ratio 0.1:1), freeze-dried W-TS, and vitamins (B₂, B₃, and B₁₂). 1,3-PD and 3-hydroxypropionaldehyde (3-HPA) did not inhibit 1,3-PD production during fermentation. Moreover, agitation did not improve fermentation rate or extent. Corn sugar was a suitable substitute for glucose. Fermentation was performed at both 20 and 150 L with 1,3-PD production of 2% (w/v, 20 g/L) being routinely achieved or exceeded.

Keywords: 1,3-propanediol; Lactobacillus panis PM1B; endemic microorganism; fermentation; glycerol; thin stillage.