Inorganic polyphosphate triggers upregulation of interleukin 11 in human osteoblast-like SaOS-2 cells

Biochem Biophys Res Commun. 2016 Oct 28;479(4):766-771. doi: 10.1016/j.bbrc.2016.09.137. Epub 2016 Sep 28.

Abstract

Polyphosphate (polyP) is abundant in bone but its roles in signaling and control of gene expression remain unclear. Here, we investigate the effect of extracellular polyP on proliferation, migration, apoptosis, gene and protein expression in human osteoblast-like SaOS-2 cells. Extracellular polyP promoted SaOS-2 cell proliferation, increased rates of migration, inhibited apoptosis and stimulated the rapid phosphorylation of extracellular-signal-regulated kinase (ERK) directly through basic fibroblast growth factor receptor (bFGFR). cDNA microarray revealed that polyP induced significant upregulation of interleukin 11 (IL-11) at both RNA and protein levels.

Keywords: Cell signaling; Extracellular matrix; Interleukin; Osteoblast; Phosphoryl transfer.

MeSH terms

  • Apoptosis / drug effects
  • Cell Line
  • Cell Movement / drug effects
  • Cell Proliferation / drug effects
  • Humans
  • Interleukin-11 / genetics*
  • Interleukin-11 / metabolism*
  • MAP Kinase Signaling System / drug effects
  • Oligonucleotide Array Sequence Analysis
  • Osteoblasts / cytology
  • Osteoblasts / drug effects*
  • Osteoblasts / metabolism*
  • Phosphorylation
  • Polyphosphates / pharmacology*
  • Receptor, Fibroblast Growth Factor, Type 1 / metabolism
  • Up-Regulation / drug effects

Substances

  • IL11 protein, human
  • Interleukin-11
  • Polyphosphates
  • FGFR1 protein, human
  • Receptor, Fibroblast Growth Factor, Type 1