Chitosan/Mimosa tenuiflora films as potential cellular patch for skin regeneration

Int J Biol Macromol. 2016 Dec;93(Pt A):1217-1225. doi: 10.1016/j.ijbiomac.2016.09.083. Epub 2016 Sep 28.

Abstract

Bio-composites films were prepared by casting and drying of aqueous solutions containing different weight ratios of chitosan and bark of Mimosa tenuiflora. The physico-chemical and functional properties of the films were characterized by scanning electron microscopy, dynamical mechanical analysis, wettability, cytotoxicity and in vitro antibacterial activities. The morphology studies confirmed that the presence of Mimosa tenuiflora change the surface of films. Moreover, the incorporation of Mimosa tenuiflora improved the thermal stability of the films, as it was indicated by the changes in the glass temperatures obtained. Water-uptake ability changed in relation to polymeric composition of film. This property increased by the addition of Mimosa tenuiflora to the film. Improved antibacterial properties were measured against Escherichia Coli and Micrococcus lysodeikticus or luteus. Finally, cytotoxicity was studied by MTT assay and the films were non-toxic. These preliminary results provide a cheap way to prepare chitosan/Mimosa tenuiflora films for wound healing and skin regeneration.

Keywords: Chitosan; Film; Mimosa tenuiflora; Skin.

MeSH terms

  • 3T3 Cells
  • Animals
  • Anti-Bacterial Agents / administration & dosage
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology
  • Anti-Bacterial Agents / toxicity
  • Bandages
  • Biocompatible Materials / administration & dosage
  • Biocompatible Materials / chemistry
  • Biocompatible Materials / pharmacology
  • Biocompatible Materials / toxicity
  • Chitosan / administration & dosage
  • Chitosan / chemistry*
  • Chitosan / pharmacology*
  • Chitosan / toxicity
  • Escherichia coli / drug effects
  • Mice
  • Micrococcus / drug effects
  • Mimosa / chemistry*
  • Regeneration / drug effects*
  • Skin / drug effects*
  • Transdermal Patch*
  • Wettability

Substances

  • Anti-Bacterial Agents
  • Biocompatible Materials
  • Chitosan