Identification of a novel canine parvovirus type 2c in Taiwan

Shu-Yun Chiang; Hung-Yi Wu; Ming-Tang Chiou; Min-Chen Chang; Chao-Nan Lin

doi:10.1186/s12985-016-0620-5

Identification of a novel canine parvovirus type 2c in Taiwan

Virol J. 2016 Sep 23;13(1):160. doi: 10.1186/s12985-016-0620-5.

Authors

Shu-Yun Chiang¹, Hung-Yi Wu², Ming-Tang Chiou^{1

3}, Min-Chen Chang¹, Chao-Nan Lin^{4

5}

Affiliations

¹ Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan.
² Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan.
³ Animal Disease Diagnostic Center, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan.
⁴ Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan. cnlin6@mail.npust.edu.tw.
⁵ Animal Disease Diagnostic Center, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan. cnlin6@mail.npust.edu.tw.

Abstract

Background: Taiwan has been considered free from canine parvovirus type 2c (CPV-2c) based on the last report of canine parvovirus type 2 (CPV-2) surveillance. However, since January 2015, the first report of CPV-2c in a puppy has occurred in Taiwan. There is currently limited information about the CPV-2c variant in Taiwan. In the present study, we characterized the previously unidentified CPV-2c variant and investigated the distribution of CPV-2 variants in Taiwan.

Methods: During January 2014 to April 2016, fecal or rectal swab samples from 99 dogs with suspected CPV-2 infection in Taiwan were collected. Eighty-eight were identified as being either CPV-2a, -2b or -2c variants positive by real-time PCR and sequence analysis.

Results: Sequence analysis of the 88 isolates confirmed CPV-2c as the dominant variant (54.6 %), followed by CPV-2b (26.1 %) and CPV-2a (19.3 %). Phylogenetic analysis demonstrated that the recent CPV-2c variants are similar to the Chinese CPV-2c strain but can be considered as novel Asian CPV-2c isolates.

Conclusion: The present study provides evidence for the existence of a novel CPV-2c variant in Taiwan.

Keywords: CPV-2c; Canine parvovirus; Taiwan.