Novel blood-brain barrier-permeable spin probe for in vivo electron paramagnetic resonance imaging

Xiaolei Wang; Miho Emoto; Yusuke Miyake; Kaori Itto; Shu Xu; Hirotada Fujii; Hiroshi Hirata; Hirokazu Arimoto

doi:10.1016/j.bmcl.2016.09.010

Novel blood-brain barrier-permeable spin probe for in vivo electron paramagnetic resonance imaging

Bioorg Med Chem Lett. 2016 Oct 15;26(20):4947-4949. doi: 10.1016/j.bmcl.2016.09.010. Epub 2016 Sep 6.

Authors

Xiaolei Wang¹, Miho Emoto², Yusuke Miyake³, Kaori Itto¹, Shu Xu¹, Hirotada Fujii², Hiroshi Hirata³, Hirokazu Arimoto⁴

Affiliations

¹ Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan.
² Department of Arts and Sciences, Center for Medical Education, Sapporo Medical University, South 1, West 17, Chuo-ku, Sapporo 060-8556, Japan.
³ Division of Bioengineering and Bioinformatics, Graduate School of Information Science and Technology, Hokkaido University, North 14, West 9, Kita-ku, Sapporo 060-0814, Japan.
⁴ Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan. Electronic address: arimoto@biochem.tohoku.ac.jp.

PMID: 27634198
DOI: 10.1016/j.bmcl.2016.09.010

Abstract

A novel blood-brain barrier (BBB)-permeable compound 10 was discovered, wherein the nitroxide moiety was linked to a nicotine acetylcholine receptor ligand. It was applied as a probe for electron paramagnetic resonance (EPR) imaging of the mouse brain. The results demonstrated that the newly synthesized compound 10 exhibited BBB permeability. These findings provide an essential discovery for in vivo EPR imaging.

Keywords: Blood–brain barrier permeable; EPR brain imaging; Nitroxide; Spin probe.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blood-Brain Barrier*
Electron Spin Resonance Spectroscopy / methods*
Mice
Spin Labels*

Substances

Spin Labels