Ethnopharmacological relevance: Corydalis bungeana Turcz. (C. bungeana) is one of traditionally used medicines in China and possesses various biological effects, such as anti-inflammatory, antibacterial activity and inhibition of the immune function of the host.
Aim of the study: we studied the anti-inflammatory effect and molecular mechanism involved of C. bungeana both in vitro and in vivo model system in which the inflammatory response was induced by LPS treatment.
Materials and methods: Anti-inflammatory activity of C. bungeana was investigated by LPS-induced RAW 264.7 macrophages and BALB/c mice. The production and expression of pro-inflammatory cytokines were evaluated by Griess reagent, ELISA kits and RT-qPCR, respectively. Phosphorylation status of IκBα and p65 was illustrated by western blot assay.
Results: C. bungeana reduced the secretion of NO, TNF-α, IL-6 and IL-1β through inhibiting the protein expression of iNOS, TNF-α, IL-6 and IL-1β in vitro and in vivo. Western blot analysis suggested that C. bungeana supressed NF-κB activation via regulating the phosphorylation of IκBα and p65. Immunohistochemical assay also demostrated the histological inflammatory change in liver tissue.
Conclusions: The results indicate that C. bungeana supresses the activation of NF-κB signaling pathway through inhibiting phosphorylation of IκBα and p65, which results in good anti-inflammatory effect. In addition, C. bungeana attenuates inflammatory reaction by supressing the expression of various inflammatory cytokines both in vivo and in vitro.
Keywords: Acetonitrile (PubChem CID: 6342); Acetylcorynoline (PubChem CID: 177015); Anti-inflammatory; C. bungeana; Corynoline (PubChem CID: 177014); DMSO (PubChem CID: 679); Dihydrosanguinarine (PubChem CID: 124069); EtOH (PubChem CID: 702); NF-κB; NaNO(2) (PubChem CID: 23668193); Pro-inflammatory cytokines; Protopine (PubChem CID: 4970); Triethylamine (PubChem CID: 8471).
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