The genus Gonothrombium is reassessed, Gonothrombium oudemansianum (Feider, 1948) redescribed and Gonothrombium ozkani sp. nov. described. All larvae were obtained by experimental rearing from field-collected females. A key to species, for adults and larvae, is provided. The distribution of the genus, hitherto known from Africa and Romania, is extended to the territory of Turkey.