The adipose tissue of origin influences the biological potential of human adipose stromal cells isolated from mediastinal and subcutaneous fat depots

Camilla Siciliano; Antonella Bordin; Mohsen Ibrahim; Isotta Chimenti; Francesco Cassiano; Ilenia Gatto; Giorgio Mangino; Andrea Coccia; Selenia Miglietta; Daniela Bastianelli; Vincenzo Petrozza; Antonella Calogero; Giacomo Frati; Elena De Falco

doi:10.1016/j.scr.2016.07.010

The adipose tissue of origin influences the biological potential of human adipose stromal cells isolated from mediastinal and subcutaneous fat depots

Stem Cell Res. 2016 Sep;17(2):342-351. doi: 10.1016/j.scr.2016.07.010. Epub 2016 Jul 30.

Authors

Affiliations

¹ Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: cami.siciliano@gmail.com.
² Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: anto.bordin@libero.it.
³ Division of Thoracic Surgery, Department of Medical-Surgical Science and Translational Medicine, Sapienza University of Rome, S. Andrea Hospital, via di Grottarossa 1035, 00189 Rome, Italy. Electronic address: mohsen.ibrahim@uniroma1.it.
⁴ Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: isotta.chimenti@uniroma1.it.
⁵ Department of Anatomy, Histology, Forensic Medicine and Orthopaedics, Section of Anatomy, Electron Microscopy Unit, Laboratory "Pietro M. Motta", Sapienza University of Rome, Rome, Italy. Electronic address: fra.cassiano@gmail.com.
⁶ Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: ilenia.gatto@uniroma1.it.
⁷ Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: gmangino@libero.it.
⁸ Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: andrea.coccia@uniroma1.it.
⁹ Department of Anatomy, Histology, Forensic Medicine and Orthopaedics, Section of Anatomy, Electron Microscopy Unit, Laboratory "Pietro M. Motta", Sapienza University of Rome, Rome, Italy. Electronic address: selenia.miglietta@uniroma1.it.
¹⁰ Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: daniela.bastianelli@uniroma1.it.
¹¹ Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: vincenzo.petrozza@uniroma1.it.
¹² Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: antonella.calogero@uniroma1.it.
¹³ Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy; Department of AngioCardioNeurology, IRCCS NeuroMed, 86077 Pozzilli, IS, Italy. Electronic address: fraticello@inwind.it.
¹⁴ Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: elena.defalco@uniroma1.it.

PMID: 27614132
DOI: 10.1016/j.scr.2016.07.010

Abstract

Indirect evidence suggests that adipose tissue-derived stromal cells (ASCs) possess different physiological and biological variations related to the anatomical localization of the adipose depots. Accordingly, to investigate the influence of the tissue origin on the intrinsic properties of ASCs and to assess their response to specific stimuli, we compared the biological, functional and ultrastructural properties of two ASC pools derived from mediastinal and subcutaneous depots (thoracic compartment) by means of supplements such as platelet lysate (PL) and FBS. Subcutaneous ASCs exhibited higher proliferative and clonogenic abilities than mediastinal counterpart, as well as increased secreted levels of IL-6 combined with lower amount of VEGF-C. In contrast, mediastinal ASCs displayed enhanced pro-angiogenic and adipogenic differentiation properties, increased cell diameter and early autophagic processes, highlighted by electron microscopy. Our results further support the hypothesis that the origin of adipose tissue significantly defines the biological properties of ASCs, and that a homogeneric function for all ASCs cannot be assumed.

Keywords: Adipose tissue; Mesenchymal stem cells; Platelet lysate angiogenesis; Transmission electron microscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipogenesis
Aged
Antibodies, Neutralizing / immunology
Autophagy
Cell Differentiation
Cell Proliferation
Cell Transdifferentiation
Cells, Cultured
Female
Humans
Interleukin-6 / metabolism
Male
Mediastinum
Microscopy, Electron, Transmission
Middle Aged
Stromal Cells / cytology*
Stromal Cells / metabolism
Stromal Cells / ultrastructure
Subcutaneous Fat / cytology*
Vascular Endothelial Growth Factor C / immunology
Vascular Endothelial Growth Factor C / metabolism

Substances

Antibodies, Neutralizing
Interleukin-6
Vascular Endothelial Growth Factor C