Applications of CRISPR Genome Engineering in Cell Biology

Fangyuan Wang; Lei S Qi

doi:10.1016/j.tcb.2016.08.004

Applications of CRISPR Genome Engineering in Cell Biology

Trends Cell Biol. 2016 Nov;26(11):875-888. doi: 10.1016/j.tcb.2016.08.004. Epub 2016 Sep 3.

Authors

Fangyuan Wang¹, Lei S Qi²

Affiliations

¹ Sino-U.S. Center of Synthetic Biology, Shanghai Institute of Rheumatology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
² Department of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA; ChEM-H, Stanford University, Stanford, CA 94305, USA. Electronic address: stanley.qi@stanford.edu.

Abstract

Recent advances in genome engineering are starting a revolution in biological research and translational applications. The clustered regularly interspaced short palindromic repeats (CRISPR)-associated RNA-guided endonuclease CRISPR associated protein 9 (Cas9) and its variants enable diverse manipulations of genome function. In this review, we describe the development of Cas9 tools for a variety of applications in cell biology research, including the study of functional genomics, the creation of transgenic animal models, and genomic imaging. Novel genome engineering methods offer a new avenue to understand the causality between the genome and phenotype, thus promising a fuller understanding of cell biology.

Keywords: CRISPR/Cas9; CRISPRi/a; cell biology; gene editing; gene regulation.

Publication types

Review
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
CRISPR-Cas Systems / genetics*
Cell Biology*
Epigenesis, Genetic
Genetic Engineering / methods*
Genome*
Humans
RNA, Guide, CRISPR-Cas Systems / metabolism

Substances

RNA, Guide, CRISPR-Cas Systems

Grants and funding

DP5 OD017887/OD/NIH HHS/United States