Simultaneous Determination of Seven Kinds of Fungicides in Citrus Fruits by Gas Chromatograghy/Mass Spectrometry

Shokuhin Eiseigaku Zasshi. 2016;57(4):101-6. doi: 10.3358/shokueishi.57.101.

Abstract

A simple and accurate procedure was developed for the determination of seven fungicides, azoxystrobin (AZO), diphenyl (DP), fludioxonil (FLUDI), imazalil (IMZ), o-phenylphenol (OPP), pyrimethanil (PYRI) and thiabendazole (TBZ), in citrus fruits. The citrus fruit sample was extracted with acetonitrile and cleaned up with a graphite carbon/aminopropyl silanized silica gel solid-phase extraction cartridge using acetonitrile-toluene (3 : 1, v/v) as the eluent. Triphenylene was used as an internal standard (I.S.) at the concentration of 0.5 μg/mL. The sample solution was subjected to GC-MS utilizing the matrix-matched standard solution method. The recoveries of AZO, FLUDI, IMZ, OPP, PYRI and TBZ spiked in domestic citrus fruits (Satsuma mandarin) at the level of 0.01-10.0 μg/g were 72.8-104% and the limits of quantification were 0.01 μg/g. The recoveries of DP spiked in domestic citrus fruits at the level of 0.01-70.0 μg/g were 70.8-80.4% and the limit of quantification was 0.01 μg/g. The proposed method was applied to the determination of fungicides in citrus fruits purchased in various markets.

MeSH terms

  • Biphenyl Compounds / analysis
  • Biphenyl Compounds / isolation & purification
  • Citrus / chemistry*
  • Dioxoles / analysis
  • Dioxoles / isolation & purification
  • Food Analysis / methods*
  • Fungicides, Industrial / analysis*
  • Fungicides, Industrial / isolation & purification
  • Gas Chromatography-Mass Spectrometry / methods*
  • Imidazoles / analysis
  • Imidazoles / isolation & purification
  • Methacrylates / analysis
  • Methacrylates / isolation & purification
  • Pyrimidines / analysis
  • Pyrimidines / isolation & purification
  • Pyrroles / analysis
  • Pyrroles / isolation & purification
  • Strobilurins
  • Thiabendazole / analysis
  • Thiabendazole / isolation & purification

Substances

  • Biphenyl Compounds
  • Dioxoles
  • Fungicides, Industrial
  • Imidazoles
  • Methacrylates
  • Pyrimidines
  • Pyrroles
  • Strobilurins
  • diphenyl
  • pyrimethanil
  • enilconazole
  • 2-phenylphenol
  • fludioxonil
  • Thiabendazole
  • azoxystrobin