New marine sterols from an algal-bearing gorgonian coral Pinnigorgia sp

Yu-Chia Chang; Nan-Fu Chen; Tsong-Long Hwang; Chung-Chih Tseng; Tung-Ying Wu; Bo-Rong Peng; Zhi-Hong Wen; Lee-Shing Fang; Yang-Chang Wu; Jyh-Horng Sheu; Ping-Jyun Sung

doi:10.1016/j.steroids.2016.08.018

New marine sterols from an algal-bearing gorgonian coral Pinnigorgia sp

Steroids. 2016 Nov:115:123-129. doi: 10.1016/j.steroids.2016.08.018. Epub 2016 Aug 21.

Authors

Yu-Chia Chang¹, Nan-Fu Chen², Tsong-Long Hwang³, Chung-Chih Tseng⁴, Tung-Ying Wu⁵, Bo-Rong Peng⁶, Zhi-Hong Wen⁷, Lee-Shing Fang⁸, Yang-Chang Wu⁹, Jyh-Horng Sheu¹⁰, Ping-Jyun Sung¹¹

Affiliations

¹ Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University and Academia Sinica, Kaohsiung 804, Taiwan; National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan.
² Division of Neurosurgery, Department of Surgery, Kaohsiung Armed Forces General Hospital, Kaohsiung 802, Taiwan.
³ Graduate Institute of Natural Products, School of Traditional Chinese Medicine, College of Medicine and Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University, Taoyuan 333, Taiwan; Research Center for Industry of Human Ecology and Graduate Institute of Health Industry Technology, Chang Gung University of Science and Technology, Taoyuan 333, Taiwan.
⁴ Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan; Division of Dentistry, Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung 813, Taiwan.
⁵ Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan.
⁶ National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan; Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944, Taiwan.
⁷ Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University and Academia Sinica, Kaohsiung 804, Taiwan; Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
⁸ Department of Sport, Health, and Leisure, Cheng Shiu University, Kaohsiung 833, Taiwan.
⁹ Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan; School of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan; Center for Molecular Medicine, China Medical University Hospital, Taichung 404, Taiwan; Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan. Electronic address: yachwu@mail.cmu.edu.tw.
¹⁰ Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University and Academia Sinica, Kaohsiung 804, Taiwan; Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan. Electronic address: sheu@mail.nsysu.edu.tw.
¹¹ National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan; Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan; Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan; Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944, Taiwan; Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan. Electronic address: pjsung@nmmba.gov.tw.

PMID: 27553726
DOI: 10.1016/j.steroids.2016.08.018

Abstract

Four new marine sterols, (22E,24R)-ergosta-5,22-diene-3β,11α-diol (1), (24S)-ergosta-5-ene-3β,11α-diol (2), 5α,6α-epoxy-23-demethylgorgost-8-ene-3β,7α-diol (3), and 5α,6α-epoxy-23-demethylgorgost-8(14)-ene-3β,7α-diol (4), along with a known metabolite, 23-demethylgorgost-7-ene-3β,5α,6β-triol (5), were isolated from an algal-bearing gorgonian coral Pinnigorgia sp., collected off the waters of Taiwan. The structures of these sterols were elucidated on the basis of spectroscopic methods. Sterols 1-5 were tested for in vitro cytotoxicity in hepatic stellate cells (HSCs). Proliferation of HSCs plays a key role in the pathogenesis of liver fibrosis.

Keywords: Gorgonian; HSCs; Marine sterol; Pinnigorgia.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anthozoa / chemistry*
Anthozoa / microbiology*
Magnetic Resonance Spectroscopy
Molecular Structure
Sterols / chemistry*

Substances

Sterols