Identification of Enterobacteriaceae and detection of carbapenemases from positive blood cultures by combination of MALDI-TOF MS and Carba NP performed after four hour subculture in Mueller Hinton

J Microbiol Methods. 2016 Oct:129:133-135. doi: 10.1016/j.mimet.2016.08.014. Epub 2016 Aug 19.

Abstract

A new protocol for Enterobacteriaceae identification and detection of carbapenemase-producing isolates from blood cultures by combining MALDI-TOF MS and the Carba NP test has been evaluated. Bacterial identification was correct in 129 of the 130 isolates tested while the Carba NP detected 28 out of the 29 carbapenemase producers.

Keywords: Blood cultures; Carba NP; Carbapenemase detection; MALDI-TOF MS.

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / blood*
  • Bacteriological Techniques*
  • Blood Culture*
  • Carbapenems / pharmacology
  • Enterobacteriaceae / classification*
  • Enterobacteriaceae / drug effects
  • Enterobacteriaceae / enzymology*
  • Enterobacteriaceae / isolation & purification
  • Enterobacteriaceae Infections / microbiology
  • Humans
  • Sensitivity and Specificity
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
  • beta-Lactamases / blood*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Carbapenems
  • beta-Lactamases
  • carbapenemase