Technical note: Assessment of the oxygen pulse and heart rate method using respiration chambers and comparative slaughter for measuring heat production of cattle

Abstract

The objective of this study was to assess the oxygen pulse and heart rate (O₂P-HR) technique using the respiration chamber (RC) and comparative slaughter (CS) methods for measuring the heat production (HP) of crossbred (Holstein × Gyr) yearling bulls. Twenty-four bulls were used. Six bulls were slaughtered at the beginning of the experiment as a reference group to estimate the initial empty body weight (BW) and energy content of the remaining animals. The remaining bulls were assigned to a completely randomized design with 3 levels of dry matter intake, with 6 replicates. The levels of dry matter intake were 1.2% of BW, 1.8% of BW and ad libitum, with target orts of 5%. The bulls were fed a diet consisting of 59.6% corn silage and 40.4% concentrate on a dry matter basis. The HP (kcal/BW^0.75) was measured using 3 techniques, first using O₂P-HR, followed by the RC and CS methods. The HP did not differ among assessed techniques, averaging 162.7kcal/BW^0.75. The intercepts of the linear regressions (mean ± SE) were 64.82±25.515 (H₀: intercept=0; P=0.024), 33.77±13.418 (H₀: intercept=0), and 50.02±27.495 (H₀: intercept=0) for O₂P-HR versus RC, CS versus RC, and O₂P-HR versus CS, respectively. The slopes of the linear regressions were 0.59±0.153 (H₀: slope=1), 0.88±0.081 (H₀: slope=1), and 0.62±0.155 (H₀: slope=1) for O₂P-HR versus RC, CS versus RC, and O₂P-HR versus CS, respectively. The coefficients of determination were 0.52, 0.90, and 0.52 for O₂P-HR versus RC, CS versus RC, and O₂P-HR versus CS, respectively. The concordance correlation coefficients, 0.70 and 0.68, were moderate for O₂P-HR versus RC and O₂P-HR versus CS, respectively, but high, 0.90, for CS versus RC. The between-animal coefficient of variation was greater for the O₂P-HR method (16.6%) compared with RC (7.7%) or CS (6.7%). We conclude that there was an agreement among the HP measurements detected using the assessed methods and that O₂P-HR is able to predict HP in cattle with great accuracy but only moderate precision. Therefore, the O₂P-HR method may have limitations in terms of assessing HP in low numbers of replications due to greater between-animal coefficient of variation than either the RC or CS methods.

Keywords: heart rate; heat production; oxygen pulse.