Detection and Molecular Identification of Sarcocystis rileyi (Apicomplexa: Sarcocystidae) From a Northern Shoveler (Anas clypeata) in Mexico

J Wildl Dis. 2016 Oct;52(4):931-935. doi: 10.7589/2016-01-004. Epub 2016 Aug 18.

Abstract

We detected macroscopic Sarcocystis cysts in a Northern Shoveler ( Anas clypeata ) in the Lerma Marshes, State of Mexico, Mexico in February 2014. The 5.0×2.0-mm macrocysts in the breast muscle of the duck were ovoid and yellow. Using an optical microscope, we saw parasitic forms of a Sarcocystis sp. among muscular fibers; the cysts measured 3.5×1.1 mm. The external wall had a smooth surface and the internal wall had a spongy texture. We identified the macrocysts as Sarcocystis rileyi according to sequences of the 18S rRNA gene, 28S rRNA gene, and ITS-1 region. Sarcocystosis should be considered in similar assessments in wild waterfowl in Mexico. Awareness of S. rileyi among anatids in the Lerma Marshes will contribute to more-effective conservation and management actions.

Keywords: Anas clypeata; Lerma; Mexico; Northern Shoveler; Sarcocystis rileyi; parasite.

MeSH terms

  • Animals
  • Ducks / microbiology*
  • Mexico
  • Phylogeny
  • Sarcocystis / isolation & purification*
  • Sarcocystis / pathogenicity
  • Sarcocystosis / veterinary