G Protein-Coupled Receptors Incorporated into Rehydrated Diblock Copolymer Vesicles Retain Functionality

M Gertrude Gutierrez; Farzad Jalali-Yazdi; Justin Peruzzi; Carson T Riche; Richard W Roberts; Noah Malmstadt

doi:10.1002/smll.201601540

G Protein-Coupled Receptors Incorporated into Rehydrated Diblock Copolymer Vesicles Retain Functionality

Small. 2016 Oct;12(38):5256-5260. doi: 10.1002/smll.201601540. Epub 2016 Aug 16.

Authors

M Gertrude Gutierrez¹, Farzad Jalali-Yazdi¹, Justin Peruzzi², Carson T Riche¹, Richard W Roberts³, Noah Malmstadt⁴

Affiliations

¹ Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, 925 Bloom Walk, Los Angeles, CA, 90089, USA.
² Department of Chemical Engineering, University of Virginia, 102 Engineers' Way, P.O. Box 400741, Charlottesville, VA, 22904-4741, USA.
³ Mork Family Department of Chemical Engineering & Materials Science, Department of Chemistry, Department of Molecular & Computational Biology, and USC Norris Comprehensive Cancer Center, University of Southern California, 925 Bloom Walk, Los Angeles, CA, 90089, USA.
⁴ Mork Family Department of Chemical Engineering & Materials Science, Department of Biomedical Engineering and Department of Chemistry, University of Southern California, 925 Bloom Walk, Los Angeles, CA, 90089, USA. malmstad@usc.edu.

Abstract

G protein-coupled receptor (GPCR) is incorporated into polymeric vesicles made up of diblock copolymer bilayers. Successfully incorporated GPCRs exhibit correct biased physiological orientation and respond to various ligands. After extended dehydrated storage via lyophilization and subsequent rehydration, diblock copolymer polymersomes retain their shape and incorporated GPCR retains its function.

Keywords: GPCR; bilayer vesicles; block copolymers; lyophilization; polymersomes.

MeSH terms

Fluorescence
Freeze Drying
Lipid Bilayers / chemistry
Polymers / chemistry*
Receptor, Serotonin, 5-HT1A / metabolism
Receptors, G-Protein-Coupled / metabolism*
Solutions
Unilamellar Liposomes / chemistry

Substances

Lipid Bilayers
Polymers
Receptors, G-Protein-Coupled
Solutions
Unilamellar Liposomes
Receptor, Serotonin, 5-HT1A

Grants and funding

R01 GM093279/GM/NIGMS NIH HHS/United States