Evaluation and characterization of thyroid-disrupting activities in soil samples along the Second Songhua River, China

Ecotoxicol Environ Saf. 2016 Nov:133:475-80. doi: 10.1016/j.ecoenv.2016.08.005. Epub 2016 Aug 13.

Abstract

In this study, a recombinant thyroid receptor (TR) gene yeast assay combined with Monte Carlo simulation were used to evaluate and characterize soil samples collected from Jilin (China) along the Second Songhua River, for their ant/agonist effect on TR. No TR agonistic activity was found in soils, but many soil samples exhibited TR antagonistic activities, and the bioassay-derived amiodarone hydrochloride equivalents, which was calculated based on Monte Carlo simulation, ranged from not detected (N.D.) to 35.5μg/g. Hydrophilic substance fractions were determined to be the contributors to TR antagonistic activity in these soil samples. Our results indicate that the novel calculation method is effective for the quantification and characterization of TR antagonists in soil samples, and these data could provide useful information for future management and remediation efforts for contaminated soils.

Keywords: In vitro bioassay; Soil sample; Thyroid receptor; Thyroid-disrupting chemicals.

Publication types

  • Evaluation Study

MeSH terms

  • Antithyroid Agents / analysis*
  • Biological Assay / methods
  • China
  • Endocrine Disruptors / analysis
  • Endocrine Disruptors / pharmacology*
  • Environmental Monitoring / methods
  • Monte Carlo Method
  • Receptors, Thyroid Hormone / genetics
  • Receptors, Thyroid Hormone / metabolism*
  • Rivers*
  • Soil / chemistry*
  • Soil Pollutants / analysis
  • Soil Pollutants / pharmacology*
  • Thyroid Gland*
  • Water Pollutants, Chemical / analysis
  • Yeasts

Substances

  • Antithyroid Agents
  • Endocrine Disruptors
  • Receptors, Thyroid Hormone
  • Soil
  • Soil Pollutants
  • Water Pollutants, Chemical