Chemical Constituents of the Rhizomes of Bletilla formosana and Their Potential Anti-inflammatory Activity

Che-Wei Lin; Tsong-Long Hwang; Fu-An Chen; Chia-Hsin Huang; Hsin-Yi Hung; Tian-Shung Wu

doi:10.1021/acs.jnatprod.6b00118

Chemical Constituents of the Rhizomes of Bletilla formosana and Their Potential Anti-inflammatory Activity

J Nat Prod. 2016 Aug 26;79(8):1911-21. doi: 10.1021/acs.jnatprod.6b00118. Epub 2016 Aug 15.

Authors

Che-Wei Lin¹, Tsong-Long Hwang^{2

3

4}, Fu-An Chen⁵, Chia-Hsin Huang⁶, Hsin-Yi Hung⁷, Tian-Shung Wu^{5

7}

Affiliations

¹ Department of Chemistry National Cheng Kung University , Tainan 70101, Taiwan.
² Graduate Institute of Natural Products, Chang Gung University , Taoyuan 33302, Taiwan.
³ Research Center for Industry of Human Ecology and Graduate Institute of Health Industry Technology, Chang Gung University of Science and Technology , Taoyuan 333, Taiwan.
⁴ Department of Anesthesiology, Chang Gung Memorial Hospital , Taoyuan 333, Taiwan.
⁵ Department of Pharmacy and Graduate Institute of Pharmaceutical Technology, Tajen University , Pingtung 90741, Taiwan.
⁶ Department of Biological Science and Technology, Chung Hwa University of Medical Technology , Tainan 71703, Taiwan.
⁷ School of Pharmacy, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University , Tainan 70101, Taiwan.

PMID: 27525452
DOI: 10.1021/acs.jnatprod.6b00118

Abstract

Nine new phenanthrenes (1-9) and a new benzyl glycoside (10) together with 45 known compounds were isolated from the rhizomes of Bletilla formosana. The structures of 1-10 were elucidated primarily on the basis of their 1D and 2D NMR spectroscopic data. Most of the isolated compounds were evaluated for their anti-inflammatory activities. The results showed that IC50 values for the inhibition of superoxide anion generation and elastase release ranged from 0.2 to 6.5 μM and 0.3 to 5.7 μM, respectively. Structure-activity relationships of the isolated compounds were also investigated. The inhibitory potencies were determined as phenanthrenes > bibenzyls > biphenanthrenes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Inflammatory Agents, Non-Steroidal / chemistry
Anti-Inflammatory Agents, Non-Steroidal / isolation & purification*
Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
Bibenzyls* / chemistry
Bibenzyls* / isolation & purification
Bibenzyls* / pharmacology
Inhibitory Concentration 50
Molecular Structure
Nuclear Magnetic Resonance, Biomolecular
Orchidaceae / chemistry*
Pancreatic Elastase / drug effects
Pancreatic Elastase / metabolism
Phenanthrenes / chemistry
Phenanthrenes / isolation & purification*
Phenanthrenes / pharmacology*
Rhizome / chemistry*
Structure-Activity Relationship
Superoxides / chemistry
Taiwan

Substances

Anti-Inflammatory Agents, Non-Steroidal
Bibenzyls
Phenanthrenes
Superoxides
Pancreatic Elastase