Structure of polysaccharide moiety of Pseudomonas xanthomarina KMM 1447T lipopolysaccharide

Maxim S Kokoulin; Svetlana V Tomshich; Anatoly I Kalinovsky; Lyudmila A Romanenko; Nadezhda A Komandrova

doi:10.1016/j.carres.2016.07.020

Structure of polysaccharide moiety of Pseudomonas xanthomarina KMM 1447^T lipopolysaccharide

Carbohydr Res. 2016 Nov 3:434:6-11. doi: 10.1016/j.carres.2016.07.020. Epub 2016 Jul 30.

Authors

Maxim S Kokoulin¹, Svetlana V Tomshich², Anatoly I Kalinovsky², Lyudmila A Romanenko², Nadezhda A Komandrova²

Affiliations

¹ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022, Vladivostok, Russian Federation. Electronic address: maxchem@mail.ru.
² G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022, Vladivostok, Russian Federation.

PMID: 27509200
DOI: 10.1016/j.carres.2016.07.020

Abstract

The structural analysis of a polysaccharide moiety of Pseudomonas xanthomarina KMM 1447^T lipopolysaccharide (LPS) was carried out. Mild acid degradation of LPS resulted in identification of two polysaccharides. The major one was built of β-D-GlcpNAcA residues amidated with L-Ala and Gly residues randomly. The minor polysaccharide was composed of branched tetrasaccharide repeating units constituted by two D-Galр, D-GalpNAc and ether of D-Glc with (2R,4R)-2,4-dihydroxypentanoic acid lactone (2R,4R-Dhpl): →3)-α-D-Galр-(1 → 3)-β-D-Galр-(1 → 3)-[β-D-Glcp4(2R,4R-Dhpl)-(1 → 4)]-β-D-GalpNAc-(1 → .

Keywords: Halocynthia aurantium; Lipopolysaccharide; Marine environment; Polysaccharide; Pseudomonas xanthomarina.

MeSH terms

Carbohydrate Sequence
Lipopolysaccharides / chemistry*
Magnetic Resonance Spectroscopy
Pseudomonas / chemistry
Pseudomonas / metabolism*

Substances

Lipopolysaccharides