Abstract
Heart ischemia is a hypoxia related disease. NOX2 and HIF-1α proteins were increased in cardiomyocytes after acute myocardial infarction. However, the relationship of the hypoxia-induced HIF-1α. NOX2-derived oxidative stress and apoptosis in cardiomyocyte remains unclear. In the current study, we use NOX2 antisense strategy to investigate the role of NOX2 in hypoxia-induced oxidative stress and apoptosis in rat cardiomyocytes. Here, we show that transduction of ADV-NOX2-AS induces potent silencing of NOX2 in cardiomyocytes, and resulting in attenuation of hypoxia-induced oxidative stress and apoptosis. This study indicates the potential of antisense-based therapies and validates NOX2 as a potent therapeutic candidate for heart ischemia.
Keywords:
Heart infarction; Heart ischemia; NOX2; Oxidative stress; and apoptosis.
MeSH terms
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Animals
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Apoptosis / genetics
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Cell Hypoxia / genetics
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Disease Models, Animal
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Humans
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Hypoxia-Inducible Factor 1, alpha Subunit / genetics
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Membrane Glycoproteins / antagonists & inhibitors
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Membrane Glycoproteins / genetics*
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Myocardial Infarction / genetics*
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Myocardial Infarction / pathology
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Myocardial Infarction / therapy
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Myocardial Reperfusion Injury / genetics*
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Myocardial Reperfusion Injury / pathology
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Myocardial Reperfusion Injury / therapy
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Myocytes, Cardiac / metabolism
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Myocytes, Cardiac / pathology
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NADPH Oxidase 2
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NADPH Oxidases / antagonists & inhibitors
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NADPH Oxidases / genetics*
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Oligonucleotides, Antisense / isolation & purification*
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Oligonucleotides, Antisense / metabolism
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Oligonucleotides, Antisense / therapeutic use
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Oxidative Stress / genetics
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Rats
Substances
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Hif1a protein, rat
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Hypoxia-Inducible Factor 1, alpha Subunit
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Membrane Glycoproteins
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Oligonucleotides, Antisense
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Cybb protein, rat
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NADPH Oxidase 2
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NADPH Oxidases