Reconstitution of intracellular environments in vitro and in artificial cells

Kei Fujiwara; Miho Yanagisawa; Shin-Ichiro M Nomura

doi:10.2142/biophysics.10.43

Reconstitution of intracellular environments in vitro and in artificial cells

Biophysics (Nagoya-shi). 2014 Aug 7:10:43-8. doi: 10.2142/biophysics.10.43. eCollection 2014.

Authors

Kei Fujiwara¹, Miho Yanagisawa², Shin-Ichiro M Nomura³

Affiliations

¹ Department of Biosciences & Informatics, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
² Department of Applied Physics, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
³ Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University, 6-6-01 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan.

Abstract

Toward reconstitution of living cells by artificial cells technology, it is critical process to understand the differences between mixtures of biomolecules and living cells. For the aim, we have developed procedures for preparation of an additive-free cell extract (AFCE) and for concentrating biomacromolecules in artificial cells. In this review, we introduce our recent progress to reconstitute intracellular environments in vitro and in artificial cells.

Keywords: Liposome; cell reconstitution; cell-free protein expression; molecular crowding.

Publication types

Review