Sphingosine-1-phosphate-enhanced Wnt5a promotes osteogenic differentiation in C3H10T1/2 cells

Yoko Hashimoto; Mari Kobayashi; Etsuko Matsuzaki; Katsumasa Higashi; Fumi Takahashi-Yanaga; Aiko Takano; Masato Hirata; Fusanori Nishimura

doi:10.1002/cbin.10652

Sphingosine-1-phosphate-enhanced Wnt5a promotes osteogenic differentiation in C3H10T1/2 cells

Cell Biol Int. 2016 Oct;40(10):1129-36. doi: 10.1002/cbin.10652. Epub 2016 Aug 14.

Authors

Yoko Hashimoto¹, Mari Kobayashi¹, Etsuko Matsuzaki^{2

3}, Katsumasa Higashi¹, Fumi Takahashi-Yanaga⁴, Aiko Takano¹, Masato Hirata⁵, Fusanori Nishimura¹

Affiliations

¹ Periodontal Section, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.
² Periodontal Section, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan. matsuzaki@college.fdcnet.ac.jp.
³ Section of Operative Dentistry and Endodontology, Department of Odontology, Fukuoka Dental College, Fukuoka, Japan. matsuzaki@college.fdcnet.ac.jp.
⁴ Department of Clinical Pharmacology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
⁵ Laboratory of Molecular and Cellular Biochemistry, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.

PMID: 27486054
DOI: 10.1002/cbin.10652

Abstract

In this study, we investigated the involvement of Wnt signaling in sphingosine-1-phosphate (S1P)-enhanced osteogenic differentiation of C3H10T1/2 pluripotent stem cells. We found that S1P enhanced the expression of Wnt5a and low-density lipoprotein receptor-related protein 5 or 6 (LRP5/6) during osteogenic differentiation. Wnt5a-neutralizing antibody inhibited S1P-enhanced expression of LRP5/6 and alkaline phosphatase, which are essential for osteogenic differentiation. Conversely, S1P did not affect endogenous canonical Wnt signaling. Taken together, S1P-enhanced Wnt5a promotes LRP5/6 expression, resulting in the trigger of osteogenic differentiation of C3H10T1/2 cells. These findings suggest a potential beneficial role for S1P in bone regeneration.

Keywords: MSC differentiation; S1P; Wnt signaling; osteoblast.

MeSH terms

Animals
Bone Regeneration
Cell Differentiation / drug effects
Cell Line
Cell Proliferation / drug effects
Intercellular Signaling Peptides and Proteins / metabolism
Low Density Lipoprotein Receptor-Related Protein-5 / biosynthesis
Low Density Lipoprotein Receptor-Related Protein-6 / biosynthesis
Lysophospholipids / pharmacology*
Mesenchymal Stem Cells / cytology
Mesenchymal Stem Cells / drug effects*
Mice
Mice, Inbred C3H
Osteoblasts / cytology
Osteoblasts / drug effects*
Osteoblasts / metabolism
Osteogenesis / drug effects
Sphingosine / analogs & derivatives*
Sphingosine / pharmacology
Wnt Signaling Pathway / drug effects
Wnt-5a Protein / genetics
Wnt-5a Protein / metabolism*

Substances

Dkk1 protein, mouse
Intercellular Signaling Peptides and Proteins
Low Density Lipoprotein Receptor-Related Protein-5
Low Density Lipoprotein Receptor-Related Protein-6
Lrp5 protein, mouse
Lrp6 protein, mouse
Lysophospholipids
Wnt-5a Protein
Wnt5a protein, mouse
sphingosine 1-phosphate
Sphingosine