Comparison of in vitro digestion model with in vivo relative bioavailability of BDE-209 in indoor dust and combination of in vitro digestion/Caco-2 cell model to estimate the daily intake of BDE-209 via indoor dust

Weijian Pan; Yuan Kang; Lixuan Zeng; Qiuyun Zhang; Jiwen Luo; Ming Hung Wong

doi:10.1016/j.envpol.2016.07.029

Comparison of in vitro digestion model with in vivo relative bioavailability of BDE-209 in indoor dust and combination of in vitro digestion/Caco-2 cell model to estimate the daily intake of BDE-209 via indoor dust

Environ Pollut. 2016 Nov:218:497-504. doi: 10.1016/j.envpol.2016.07.029. Epub 2016 Jul 29.

Authors

Weijian Pan¹, Yuan Kang², Lixuan Zeng¹, Qiuyun Zhang¹, Jiwen Luo¹, Ming Hung Wong³

Affiliations

¹ School of Chemistry and Environment, South China Normal University, Higher Education Mega Center, Guangzhou 510006, People's Republic of China; Guangdong Engineering Technology Research Center for Drinking Water Safety, School of Chemistry and Environment, South China Normal University, Higher Education Mega Center, Guangzhou 510006, People's Republic of China.
² School of Chemistry and Environment, South China Normal University, Higher Education Mega Center, Guangzhou 510006, People's Republic of China; Guangdong Engineering Technology Research Center for Drinking Water Safety, School of Chemistry and Environment, South China Normal University, Higher Education Mega Center, Guangzhou 510006, People's Republic of China. Electronic address: kangyuan@m.scnu.edu.cn.
³ Consortium on Health, Environment, Education and Research (CHEER), and Department of Science and Environmental Studies, Hong Kong Institute of Education, Hong Kong, People's Republic of China; School of Environment, Jinan University, Guangzhou, People's Republic of China.

PMID: 27476428
DOI: 10.1016/j.envpol.2016.07.029

Abstract

There is limited information on the BDE-209 relative bioavailability (RBA) of indoor dust and the absorption of BDE-209 after in vitro digestion was seldom studied. In the present study, BDE-209 RBA in 6 household dust samples measured using an in vivo mouse model was compared to BDE-209 bioaccessibility determined using physiologically based extraction test (PBET) and solubility bioaccessibility research consortium method (SBRC) assays. BDE-209 RBA obtained ranged from 45.9 ± 16.1 to 96.0 ± 17.4% and exhibited a significant relationship with PBET gastric phase (r² = 0.578, p = 0.080), small intestinal phase (r² = 0.728, p = 0.031) and total BDE-209 bioaccessibility (r² = 0.728, p = 0.031), which indicated PBET assay can serve as a surrogate to predict BDE-209 RBA to refine human health exposure. In addition, the absorption of BDE-209 by Caco-2 cell line was assessed. With the consideration of the corresponding bioaccessibility and absorption of BDE-209 by Caco-2 cell line, the human daily intake of BDE-209 via dust ingestion for adults and children was much lower than that estimated by total concentration.

Keywords: BDE-209; Caco-2 cells; In vitro digestion; Relative bioavailability.

MeSH terms

Animals
Biological Assay
Biological Availability
Caco-2 Cells
Dust / analysis*
Environmental Monitoring / methods
Environmental Pollutants / analysis
Environmental Pollutants / metabolism*
Female
Halogenated Diphenyl Ethers / analysis
Halogenated Diphenyl Ethers / metabolism*
Humans
Mice
Solubility

Substances

Dust
Environmental Pollutants
Halogenated Diphenyl Ethers
decabromobiphenyl ether