Isolation and identification of cellulose-producing strain Komagataeibacter intermedius from fermented fruit juice

Shin-Ping Lin; Yin-Hsuan Huang; Kai-Di Hsu; Ying-Jang Lai; Yu-Kuo Chen; Kuan-Chen Cheng

doi:10.1016/j.carbpol.2016.06.032

Isolation and identification of cellulose-producing strain Komagataeibacter intermedius from fermented fruit juice

Carbohydr Polym. 2016 Oct 20:151:827-833. doi: 10.1016/j.carbpol.2016.06.032. Epub 2016 Jun 7.

Authors

Shin-Ping Lin¹, Yin-Hsuan Huang², Kai-Di Hsu³, Ying-Jang Lai⁴, Yu-Kuo Chen⁵, Kuan-Chen Cheng⁶

Affiliations

¹ Institute of Biotechnology, National Taiwan University, Taipei 10617, Taiwan. Electronic address: d00642009@ntu.edu.tw.
² Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan. Electronic address: b02603033@ntu.edu.tw.
³ Institute of Biotechnology, National Taiwan University, Taipei 10617, Taiwan. Electronic address: twhsu72@gmail.com.
⁴ Department of Food Science, National Quemoy University, Kinmen 89250, Taiwan. Electronic address: d91641006@gmail.com.
⁵ Department of Food Science, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan. Electronic address: chenyk@mail.npust.edu.tw.
⁶ Institute of Biotechnology, National Taiwan University, Taipei 10617, Taiwan; Graduate Institute of Food Science Technology, National Taiwan University, Taipei 10617, Taiwan; Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan. Electronic address: kccheng@ntu.edu.tw.

PMID: 27474630
DOI: 10.1016/j.carbpol.2016.06.032

Abstract

A bacterial cellulose (BC) producing strain isolated from fermented fruit juice was identified as Komagataeibacter intermedius (K. intermedius) FST213-1 by 16s rDNA sequencing analysis and biochemical characteristics test. K. intermedius FST213-1 can produce BC within pH 4-9 and exhibit maximum BC production (1.2g/L) at pH 8 in short-term (4-day) cultivation. Results of Fourier transform infrared spectroscopy, X-ray diffraction, water content, thermogravimetric analysis and mechanical property indicated that BC produced from K. intermedius FST213-1 exhibits higher water content ability (99.5%), lower thermostability (315°C), lower crystallinity (79.3%) and similar mechanical properties in comparison with the specimen from model BC producer, Gluconacetobacter xylinus 23769. Based on these analyses, the novel based-resistant strain K. intermedius FST213-1 can efficiently produce BC, which can be applied for industrial manufacturing with potential features.

Keywords: Bacterial cellulose; Base-resistant strain; Komagataeibacter intermedius; Materials property analysis; Strain selection.

MeSH terms

Acetobacteraceae / isolation & purification*
Acetobacteraceae / metabolism*
Cellulose / biosynthesis*
Fermentation*
Fruit and Vegetable Juices / microbiology*
Hydrogen-Ion Concentration
Temperature

Substances

Cellulose