A functional chitosan membrane with grafted epigallocatechin-3-gallate and lovastatin enhances periodontal tissue regeneration in dogs

Bor-Shiunn Lee; Chien-Chen Lee; Hung-Pin Lin; Wei-An Shih; Wan-Ling Hsieh; Chern-Hsiung Lai; Yasuo Takeuchi; Yi-Wen Chen

doi:10.1016/j.carbpol.2016.06.026

A functional chitosan membrane with grafted epigallocatechin-3-gallate and lovastatin enhances periodontal tissue regeneration in dogs

Carbohydr Polym. 2016 Oct 20:151:790-802. doi: 10.1016/j.carbpol.2016.06.026. Epub 2016 Jun 6.

Authors

Bor-Shiunn Lee¹, Chien-Chen Lee², Hung-Pin Lin³, Wei-An Shih⁴, Wan-Ling Hsieh⁵, Chern-Hsiung Lai⁶, Yasuo Takeuchi⁷, Yi-Wen Chen⁸

Affiliations

¹ Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University and National Taiwan University Hospital, Taipei 10048, Taiwan. Electronic address: leebs@ntu.edu.tw.
² Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University and National Taiwan University Hospital, Taipei 10048, Taiwan. Electronic address: zionist35@hotmail.com.
³ Department of Dentistry, MacKay Memorial Hospital, Taipei 10449, Taiwan; Department of Dentistry, School of Dentistry, National Taiwan University, Taipei 10048, Taiwan. Electronic address: hungpin@gmail.com.
⁴ Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University and National Taiwan University Hospital, Taipei 10048, Taiwan. Electronic address: v79319@hotmail.com.
⁵ Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University and National Taiwan University Hospital, Taipei 10048, Taiwan. Electronic address: r00450017@ntu.edu.tw.
⁶ College of Life Science, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. Electronic address: chern@kmu.edu.tw.
⁷ Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo 113-8549, Japan. Electronic address: takeuchi.peri@tmd.ac.jp.
⁸ Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University and National Taiwan University Hospital, Taipei 10048, Taiwan. Electronic address: yiwenchen@ntu.edu.tw.

PMID: 27474626
DOI: 10.1016/j.carbpol.2016.06.026

Abstract

Currently used guided tissue regeneration (GTR) membranes are mainly used as a barrier to prevent epithelial cells growth into defects before new bone formation. The aim of this study was to develop a tri-layer functional chitosan (CS) membrane with epigallocatechin-3-gallate (EGCG) grafted on the outer layer for bactericidal activity, and lovastatin was included in the middle layer for controlled release. Successful EGCG grafting was demonstrated using Fourier transform infrared spectroscopy and EGCG grafting significantly enhanced adhesion and proliferation of human gingival fibroblasts. The release duration of lovastatin reached 21days. CS-Lovastatin1 produced the highest alkaline phosphatase activity and EGCG14-CS exhibited the best bactericidal activity against periodontopathic bacteria. Finally, the EGCG14-CS-Lovastatin1 membrane showed a higher percentage of bone regeneration than BioMend(®) and control groups in one-walled defects of beagle dogs. These results suggest that the EGCG14-CS-Lovastatin1 membrane has the potential to be used as a novel GTR membrane.

Keywords: Chitosan; Collagen membrane; Epigallocatechin-3-gallate; Guided tissue regeneration; Lovastatin; Periodontitis.

MeSH terms

Alkaline Phosphatase / metabolism
Animals
Anti-Bacterial Agents / chemistry*
Anti-Bacterial Agents / pharmacology
Anti-Bacterial Agents / toxicity
Catechin / analogs & derivatives*
Catechin / chemistry
Cell Adhesion / drug effects
Cell Proliferation / drug effects
Chitosan / chemistry*
Delayed-Action Preparations
Dogs
Drug Carriers / chemistry*
Drug Carriers / pharmacology
Drug Carriers / toxicity
Drug Liberation
Fibroblasts / cytology
Fibroblasts / drug effects
Fibroblasts / metabolism
Gingiva / cytology
Gingiva / drug effects
Guided Tissue Regeneration*
Humans
Lovastatin / chemistry*
Male
Membranes, Artificial*
Tensile Strength

Substances

Anti-Bacterial Agents
Delayed-Action Preparations
Drug Carriers
Membranes, Artificial
Catechin
Chitosan
Lovastatin
epigallocatechin gallate
Alkaline Phosphatase