Effects of abalone (Haliotis discus hannai Ino) gonad polysaccharides on cholecystokinin release in STC-1 cells and its signaling mechanism

Jun Zhao; Da-Yong Zhou; Jing-Feng Yang; Shuang Song; Ting Zhang; Ce Zhu; Yan-Qing Song; Chen-Xu Yu; Bei-Wei Zhu

doi:10.1016/j.carbpol.2016.05.047

Effects of abalone (Haliotis discus hannai Ino) gonad polysaccharides on cholecystokinin release in STC-1 cells and its signaling mechanism

Carbohydr Polym. 2016 Oct 20:151:268-273. doi: 10.1016/j.carbpol.2016.05.047. Epub 2016 May 17.

Authors

Jun Zhao¹, Da-Yong Zhou², Jing-Feng Yang², Shuang Song², Ting Zhang³, Ce Zhu³, Yan-Qing Song³, Chen-Xu Yu⁴, Bei-Wei Zhu⁵

Affiliations

¹ School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China; School of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian 116034, PR China.
² School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China; National and Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian 116034, PR China.
³ School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China.
⁴ School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China; Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA 50011, USA.
⁵ School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China; School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China; National and Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian 116034, PR China. Electronic address: zhubeiwei@163.com.

PMID: 27474567
DOI: 10.1016/j.carbpol.2016.05.047

Abstract

Abalone gonad polysaccharide (AGP) -31, -32 and -33 prepared in this study had the molecular weight (MW) of 37.8, 32.2 and 27.5kDa, respectively. They all contained mannose, rhamnose, glucuronic acid, glucose, galactose, xylose, arabinose, and fucose, with very similar monosaccharide profile. All the three polysaccharides could significantly increase the secretion of cholecystokinin (CCK) in STC-1 cells. Among them, AGP-32 showed the strongest effect. However, the low-MW fragments of AGP-32 showed significantly lower activity than AGP-32 itself. It was also found that the inhibitors on calcium-sensing receptor (CaSR), protein kinase A (PKA), Ca(2+)⁄calmodulin-dependent protein kinase (CaMK) II, p38- mitogen-activated protein kinases (MAPK), and an intracellular calcium chelator all inhibited AGP-induced CCK secretion. To conclude, Ca(2+)/calmodulin (CaM)/CaMK, cyclic adenosine monophosphate (cAMP)/PKA and MAPK pathways are all involved in AGP-induced CCK secretion.

Keywords: 3-Phenylphenol (PubChem CID: 11381); Abalone (Haliotis discus hannai Ino) gonad; Alkaline protease (PubChem CID: 72355); BAPTA-AM (PubChem CID: 2293); Cholecystokinin; Enteroendocrine STC-1 cells; H89 (PubChem CID: 449241); KN62 (PubChem CID: 3838); NPS2143 (PubChem CID: 6918446); Polysaccharides; SB203580 (PubChem CID:176155); Signaling pathway; Sodium tetraborate (PubChem CID: 13211214); Trifluoroacetic acid (PubChem CID: 6422).