Detection and Analysis of Ebola Virus in Sierra Leone-China Friendship Biosafety Laboratory from March 11 to April 20, 2015

Biomed Environ Sci. 2016 Jun;29(6):443-7. doi: 10.3967/bes2016.057.

Abstract

Ebola virus disease reemerged in Western Africa in 2014. Chinese Center for Disease Control and Prevention dispatched the first Ebola virus (EBOV) detection team to run newly established Sierra Leone-China Friendship Biological Safety Laboratory. The aims of study were to understand epidemiology, clinical manifestations and survival time of EBOV in patient's blood. A total of 913 specimens were tested between March 11 and April 20, 2015. EBOV positivity occurred in 7.37% of the blood and 0.53% in throat swabs. Most commonly reported symptoms of laboratory confirmed patients were intense fatigue, anorexia, and fever. EBOV RNAs persisted in blood for almost 4 weeks and the real-time RT-PCR Ct values showed close correlation with the sampling time after onset.

Publication types

  • Letter

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Blood / virology
  • Child
  • Child, Preschool
  • Ebolavirus / isolation & purification*
  • Hemorrhagic Fever, Ebola / blood*
  • Hemorrhagic Fever, Ebola / epidemiology
  • Hemorrhagic Fever, Ebola / virology
  • Humans
  • Infant
  • Laboratories
  • Middle Aged
  • Real-Time Polymerase Chain Reaction
  • Sierra Leone / epidemiology
  • Young Adult