Sickle Cell Imaging Flow Cytometry Assay (SIFCA)

Kleber Y Fertrin; Leigh Samsel; Eduard J van Beers; Laurel Mendelsohn; Gregory J Kato; J Philip McCoy Jr

doi:10.1007/978-1-4939-3302-0_20

Sickle Cell Imaging Flow Cytometry Assay (SIFCA)

Methods Mol Biol. 2016:1389:279-92. doi: 10.1007/978-1-4939-3302-0_20.

Authors

Kleber Y Fertrin^{1

2}, Leigh Samsel³, Eduard J van Beers⁴, Laurel Mendelsohn⁵, Gregory J Kato⁶, J Philip McCoy Jr⁷

Affiliations

¹ Department of Clinical Pathology, School of Medical Sciences, University of Campinas, Campinas, Brazil.
² Hematology and Hemotherapy Center-Hemocentro Campinas, University of Campinas, Campinas, Brazil.
³ NHLBI Flow Cytometry Core Facility, NHLBI, NIH, Building 31, Room 5A52, MSC 2486, 31 Center Drive, Bethesda, MD, 20892, USA.
⁴ Department of Clinical Hematology, Academic Medical Centre, Amsterdam, The Netherlands.
⁵ Hematology Branch, NHLBI, NIH, Bethesda, MD, 20892, USA.
⁶ Division of Hematology/Oncology, Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA, 15261, USA.
⁷ NHLBI Flow Cytometry Core Facility, NHLBI, NIH, Building 31, Room 5A52, MSC 2486, 31 Center Drive, Bethesda, MD, 20892, USA. mccoyj@nhlbi.nih.go.

Abstract

Hemoglobin S polymerization under hypoxic conditions in sickle cell disorders causes characteristic shape changes to human red blood cells. Previous sickling assays used to investigate the efficacy of novel agents to treat these disorders are laborious and observer dependent. Here, we describe a partially automated, high-throughput sickling assay using imaging flow cytometry.

Keywords: Imaging flow cytometry; Sickle cell disease; Sickling assay.

Publication types

Research Support, N.I.H., Intramural

MeSH terms

Anemia, Sickle Cell / pathology*
Erythrocytes / pathology*
Flow Cytometry / instrumentation
Flow Cytometry / methods*
Hemoglobin, Sickle / analysis
Humans

Substances

Hemoglobin, Sickle

Abstract

Publication types

MeSH terms

Substances

Grants and funding