Gastrokine 1 mRNA in human sera is not informative biomarker for gastric cancer

J Negat Results Biomed. 2016 Jul 25;15(1):14. doi: 10.1186/s12952-016-0057-9.

Abstract

Background: We aimed to ascertain if Gastrokine 1 mRNA in the sera of patients with gastric cancer might be an informative biomarker for the disease.

Results: Analysis of GKN1 mRNA in serum samples from healthy individuals (n = 23) and from patients with diagnosis of gastric cancer (n = 16), performed by using absolute quantification based on standard curve method, did not show any significative statistical difference between the two unpaired group of individuals.

Conclusions: Our preliminary results did not confirm GKN1 as a potential biomarker for gastric cancer.

Keywords: Absolute qRT-PCR, 18 kDa antrum mucosal protein; Gastric cancer biomarker; Gastrokine 1.

MeSH terms

  • Base Sequence
  • Biomarkers, Tumor / blood*
  • Biomarkers, Tumor / genetics*
  • Case-Control Studies
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Male
  • Middle Aged
  • Peptide Hormones / blood*
  • Peptide Hormones / genetics*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Real-Time Polymerase Chain Reaction
  • Stomach Neoplasms / blood*
  • Stomach Neoplasms / genetics*

Substances

  • Biomarkers, Tumor
  • GKN1 protein, human
  • Peptide Hormones
  • RNA, Messenger