Emergence of Mycoplasma genitalium strains showing mutations associated with macrolide and fluoroquinolone resistance in the region Dresden, Germany

Roger Dumke; Alexander Thürmer; Enno Jacobs

doi:10.1016/j.diagmicrobio.2016.07.005

Emergence of Mycoplasma genitalium strains showing mutations associated with macrolide and fluoroquinolone resistance in the region Dresden, Germany

Diagn Microbiol Infect Dis. 2016 Oct;86(2):221-3. doi: 10.1016/j.diagmicrobio.2016.07.005. Epub 2016 Jul 8.

Authors

Roger Dumke¹, Alexander Thürmer², Enno Jacobs²

Affiliations

¹ TU Dresden, Institute of Medical Microbiology and Hygiene, Dresden, Germany. Electronic address: roger.dumke@tu-dresden.de.
² TU Dresden, Institute of Medical Microbiology and Hygiene, Dresden, Germany.

PMID: 27448452
DOI: 10.1016/j.diagmicrobio.2016.07.005

Abstract

Among 323 specimens from male patients with symptoms of non-gonococcal urethritis, Mycoplasma genitalium was detected in 19 samples by real-time PCR. Mutations of 23S rRNA gene associated with macrolide resistance were confirmed in 10 strains. Amino acid changes at positions 81 and 83 of ParC protein were demonstrated indicating quinolone resistance of two strains.

Keywords: 23S rRNA mutation; Genotyping; Macrolide resistance; Mycoplasma genitalium; Quinolone resistance; Topoisomerase mutation.

MeSH terms

Anti-Bacterial Agents / pharmacology*
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Drug Resistance, Bacterial*
Fluoroquinolones / pharmacology*
Genotype
Germany
Humans
Macrolides / pharmacology*
Male
Mutation*
Mycoplasma Infections / microbiology
Mycoplasma genitalium / drug effects*
Mycoplasma genitalium / genetics
Mycoplasma genitalium / isolation & purification*
Polymerase Chain Reaction
RNA, Ribosomal, 23S / genetics
Sequence Analysis, DNA

Substances

Anti-Bacterial Agents
DNA, Bacterial
DNA, Ribosomal
Fluoroquinolones
Macrolides
RNA, Ribosomal, 23S