Lowering photosensitizer doses and increasing fluences induce apoptosis in tumor bearing mice

Katja Haedicke; Susanna Graefe; Ulf Teichgraeber; Ingrid Hilger

doi:10.1364/BOE.7.002641

Lowering photosensitizer doses and increasing fluences induce apoptosis in tumor bearing mice

Biomed Opt Express. 2016 Jun 16;7(7):2641-9. doi: 10.1364/BOE.7.002641. eCollection 2016 Jul 1.

Authors

Katja Haedicke¹, Susanna Graefe², Ulf Teichgraeber¹, Ingrid Hilger¹

Affiliations

¹ Experimental Radiology, Institute of Diagnostic and Interventional Radiology, Jena University Hospital, Friedrich-Schiller University Jena, Erlanger Allee 101, D-07747 Jena, Germany.
² Biolitec Research GmbH, Research & Development, Otto-Schott-Straße 15, D-07745 Jena, Germany.

Abstract

The objective of this study was to determine an optimal dose of photodynamic therapy (PDT) for inducing apoptotic tumor cells in vivo. In this context, mice bearing human tongue-squamous epithelium carcinomas were treated with various photosensitizer concentrations and fluences. Tumor apoptosis was imaged after 2 days via a self-designed DY-734-annexin V probe using near-infrared fluorescence (NIRF) optical imaging. Apoptosis was verified ex vivo via TUNEL staining. Apoptotic tumor cells were detected in vivo at a dose of 40 µg photosensitizer and a fluency of 100 J/cm(2). This is the lowest photosensitizer dose reported so far.

Keywords: (000.1430) Biology and medicine; (170.0110) Imaging systems; (170.0170) Medical optics and biotechnology; (170.5380) Physiology.