Dynamic full field optical coherence tomography: subcellular metabolic contrast revealed in tissues by interferometric signals temporal analysis

Clement Apelian; Fabrice Harms; Olivier Thouvenin; A Claude Boccara

doi:10.1364/BOE.7.001511

Dynamic full field optical coherence tomography: subcellular metabolic contrast revealed in tissues by interferometric signals temporal analysis

Biomed Opt Express. 2016 Mar 24;7(4):1511-24. doi: 10.1364/BOE.7.001511. eCollection 2016 Apr 1.

Authors

Clement Apelian¹, Fabrice Harms², Olivier Thouvenin³, A Claude Boccara¹

Affiliations

¹ Institut Langevin-ESPCI ParisTech, PSL Research University 1 Rue Jussieu, 75005, Paris, France; LLTech SAS, Pépinière Paris Santé Cochin 29 Rue du Faubourg Saint Jacques, 75014, Paris, France.
² LLTech SAS, Pépinière Paris Santé Cochin 29 Rue du Faubourg Saint Jacques, 75014, Paris, France.
³ Institut Langevin-ESPCI ParisTech, PSL Research University 1 Rue Jussieu, 75005, Paris, France.

Abstract

We developed a new endogenous approach to reveal subcellular metabolic contrast in fresh ex vivo tissues taking advantage of the time dependence of the full field optical coherence tomography interferometric signals. This method reveals signals linked with local activity of the endogenous scattering elements which can reveal cells where other OCT-based techniques fail or need exogenous contrast agents. We benefit from the micrometric transverse resolution of full field OCT to image intracellular features. We used this time dependence to identify different dynamics at the millisecond scale on a wide range of organs in normal or pathological conditions.

Keywords: (100.0100) Image processing; (110.4500) Optical coherence tomography; (110.6915) Time imaging; (170.1530) Cell analysis; (170.6930) Tissue; (180.6900) Three-dimensional microscopy.