Preparation and Assay of Simple Light off Biosensor Based on Immobilized Bioluminescent Bacteria for General Toxicity Assays

G V M Gabriel; V R Viviani

doi:10.1007/978-1-4939-3813-1_18

Preparation and Assay of Simple Light off Biosensor Based on Immobilized Bioluminescent Bacteria for General Toxicity Assays

Methods Mol Biol. 2016:1461:217-23. doi: 10.1007/978-1-4939-3813-1_18.

Authors

G V M Gabriel^{1

2

3}, V R Viviani^{4

5

6}

Affiliations

¹ Laboratory of Biochemistry and Biotechnology of Bioluminescence, Department of Physics, Chemistry and Mathematics, Federal University of São Carlos (UFSCar), Sorocaba, SP, Brazil.
² Graduate School of Biotechnology and Environmental Monitoring, Federal University of São Carlos (UFSCar), il. João Leme dos Santos Road, SP-264, Km 110, Sorocaba, SP, Brazil.
³ Graduate School of Evolutive Genetics and Molecular Biology, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil.
⁴ Laboratory of Biochemistry and Biotechnology of Bioluminescence, Department of Physics, Chemistry and Mathematics, Federal University of São Carlos (UFSCar), Sorocaba, SP, Brazil. viviani@ufscar.br.
⁵ Graduate School of Biotechnology and Environmental Monitoring, Federal University of São Carlos (UFSCar), il. João Leme dos Santos Road, SP-264, Km 110, Sorocaba, SP, Brazil. viviani@ufscar.br.
⁶ Graduate School of Evolutive Genetics and Molecular Biology, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil. viviani@ufscar.br.

PMID: 27424908
DOI: 10.1007/978-1-4939-3813-1_18

Abstract

We describe a simple light off bioluminescent biosensor for general environmental toxicity assays based on immobilized bioluminescent bacteria engineered with beetle luciferases.

Keywords: Bioluminescent biosensor; Toxicity biosensor; pH-sensitive firefly luciferase.

MeSH terms

Bacteria / drug effects
Bacteria / genetics*
Bacteria / metabolism*
Biosensing Techniques*
Gene Expression
Genes, Reporter
Hydrogen-Ion Concentration
Light*
Luciferases, Firefly / genetics
Luciferases, Firefly / metabolism
Luminescent Measurements / methods*
Microbial Viability / drug effects
Toxicity Tests / methods*

Substances

Luciferases, Firefly