A rat model of oral submucous fibrosis induced by bleomycin

Oral Surg Oral Med Oral Pathol Oral Radiol. 2016 Aug;122(2):216-23. doi: 10.1016/j.oooo.2015.07.042. Epub 2016 Apr 19.

Abstract

Objective: The aim of this study was to induce a rat model of oral submucous fibrosis (OSF) using bleomycin.

Study design: A randomized trial was performed. Bleomycin dilution or phosphate-buffered saline was injected daily into the buccal mucosa of the test and control rats, respectively, for 2, 4, 6, or 8 weeks. Changes in histopathologic features, myofibroblasts, ultrastructure, and the levels of collagen type I and III, transforming growth factor-beta 1, and interferon-γ were measured.

Results: Compared with controls, the buccal mucosa of the test rats appeared pale and stiff. There was collagen deposition in the laminae propria. Type I and type III collagen increased steadily, transforming growth factor-beta 1 increased and then decreased, and interferon-γ was gradually reduced. The ultrastructure was also similar to that of OSF.

Conclusions: Using bleomycin, we induced a rat model of OSF that is very similar to human OSF in clinical manifestations, pathology, and ultrastructural changes.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Animals
  • Biomarkers / metabolism
  • Bleomycin / toxicity*
  • Blotting, Western
  • Collagen / metabolism
  • Disease Models, Animal
  • Female
  • Immunoenzyme Techniques
  • Interferon-gamma / metabolism
  • Microscopy, Electron, Transmission
  • Oral Submucous Fibrosis / chemically induced*
  • Rats
  • Rats, Sprague-Dawley
  • Transforming Growth Factor beta1 / metabolism

Substances

  • Biomarkers
  • Transforming Growth Factor beta1
  • Bleomycin
  • Interferon-gamma
  • Collagen