Abstract
The search for new bioactive molecules is a driving force for research pharmaceutical industries, especially those molecules obtained from fermentation. The molecules possessing angiogenic and anti-inflammatory attributes have attracted attention and are the focus of this study. Angiogenic activity from kefir polysaccharide extract, via chorioallantoic membrane assay, exhibited a pro-angiogenic effect compared with vascular endothelial factor (pro-angiogenic) and hydrocortisone (anti-angiogenic) activity as standards with an EC50 of 192ng/mL. In terms of anti-inflammatory activity determined via hyaluronidase enzyme assay, kefir polysaccharide extract inhibited the enzyme with a minimal activity of 2.08mg/mL and a maximum activity of 2.57mg/mL. For pharmaceutical purposes, kefir polysaccharide extract is considered to be safe because it does not inhibit VERO cells in cytotoxicity assays.
Keywords:
Angiogenic activity; Anti-inflammatory activity; Hyaluronidase; Tibetan kefir.
Copyright © 2016 Elsevier Inc. All rights reserved.
MeSH terms
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Angiogenesis Inducing Agents / isolation & purification
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Angiogenesis Inducing Agents / pharmacology*
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Angiogenesis Inducing Agents / toxicity
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Animals
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Anti-Inflammatory Agents / isolation & purification
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Anti-Inflammatory Agents / pharmacology*
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Anti-Inflammatory Agents / toxicity
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Cell Survival / drug effects
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Chick Embryo
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Chickens
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Chlorocebus aethiops
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Chorioallantoic Membrane / blood supply*
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Dose-Response Relationship, Drug
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Glycoside Hydrolase Inhibitors / isolation & purification
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Glycoside Hydrolase Inhibitors / pharmacology*
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Glycoside Hydrolase Inhibitors / toxicity
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Hyaluronoglucosaminidase / antagonists & inhibitors*
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Hyaluronoglucosaminidase / metabolism
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Kefir / microbiology*
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Neovascularization, Physiologic / drug effects*
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Polysaccharides, Bacterial / isolation & purification
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Polysaccharides, Bacterial / pharmacology*
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Polysaccharides, Bacterial / toxicity
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Vero Cells
Substances
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Angiogenesis Inducing Agents
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Anti-Inflammatory Agents
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Glycoside Hydrolase Inhibitors
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Polysaccharides, Bacterial
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Hyaluronoglucosaminidase