Data on morphology, large-scale chromatin configuration and the occurrence of proteins and rRNA in nucleolus-like bodies of fully-grown mouse oocytes in different fixatives

Kseniya V Shishova; Yuriy M Khodarovich; Elena A Lavrentyeva; Olga V Zatsepina

doi:10.1016/j.dib.2016.03.085

Data on morphology, large-scale chromatin configuration and the occurrence of proteins and rRNA in nucleolus-like bodies of fully-grown mouse oocytes in different fixatives

Data Brief. 2016 Apr 1:7:1179-84. doi: 10.1016/j.dib.2016.03.085. eCollection 2016 Jun.

Authors

Kseniya V Shishova¹, Yuriy M Khodarovich¹, Elena A Lavrentyeva², Olga V Zatsepina¹

Affiliations

¹ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Street, 16/10, Moscow 117997, Russian Federation.
² Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Street, 16/10, Moscow 117997, Russian Federation; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, GSP-1, Leninskiye Gory, MSU, 1-73, Office 433, Moscow 119991, Russian Federation.

Abstract

Here we provide data on accessibility of nucleolus-like bodies (NLBs) of fully-grown (GV) mouse oocytes to fluorescence in situ hybridization (FISH) probes and anti-nucleolar antibodies as well as on oocyte general morphology and large scale chromatin configuration, which relate to the research article "High-resolution microscopy of active ribosomal genes and key members of the rRNA processing machinery inside nucleolus-like bodies of fully-grown mouse oocytes" (Shishova et al., 2015 [1]). Experimental factors include: a cross-linking reagent formaldehyde and two denaturing fixatives, such as 70% ethanol and a mixture of absolute methanol and glacial acetic acid (3:1, v/v).

Keywords: Fixation procedure; GV oocytes; Nucleolus-like bodies; Proteins; RNA.