Oct4B, CD90, and CD73 are upregulated in bladder tissue following electro-resection of the bladder

Takumi Takeuchi; Akiko Tonooka; Yumiko Okuno; Mami Hattori-Kato; Koji Mikami

doi:10.46582/jsrm.1201003

Oct4B, CD90, and CD73 are upregulated in bladder tissue following electro-resection of the bladder

J Stem Cells Regen Med. 2016 May 30;12(1):10-5. doi: 10.46582/jsrm.1201003. eCollection 2016.

Authors

Takumi Takeuchi¹, Akiko Tonooka², Yumiko Okuno¹, Mami Hattori-Kato¹, Koji Mikami¹

Affiliations

¹ Department of Urology, Kanto Rosai Hospital, Kizukisumiyoshi-cho, Nakahara-ku, Kawasaki, Japan.
² Department of Pathology, Kanto Rosai Hospital, Kizukisumiyoshi-cho, Nakahara-ku, Kawasaki, Japan.

Abstract

Aim: We tested the hypothesis that stimulation by electro-resection of bladder tissue induces stem cells in the tissue repair process.

Materials & methods: After primary transurethral resection of a bladder tumor and surrounding tissue (TUR-Bt), second TUR-Bt was performed. Tissues excised by second TUR-Bt were immunohistochemically stained for Oct4, a marker of pluripotency, and for CD90 and CD73, markers of mesenchymal stromal cells, when no bladder tumor cells remained.

Results and conclusions: Oct4B protein was sporadically stained in the cytoplasm of interstitial cells in four out of eight cases. CD90 and CD73 are upregulated in interstitial and vascular endothelial cells without CD45 expression. Mesenchymal stromal cells, but not pluripotent stem cells, may be mainly involved in bladder tissue repair.

Keywords: Bladder; CD73; CD90; Electric; Oct4B; Stimulation.