Abstract
We aimed to identify new cell-membrane antigens implicated in opsoclonus-myoclonus with neuroblastoma. The sera of 3 out of 14 patients showed IgG electron-microscopy immunogold reactivity on SH-SY5Y neuroblastoma cells. Immunoprecipitation experiments using rat brain synaptosomes and SH-SY5Y cells led to the identification of: (1) thirty-one nuclear/cytoplasmic proteins (including antigens HuB, HuC); (2) seven neuronal membrane proteins, including the Shaw-potassium channel Kv3.3 (KCNC3), whose genetic disruption in mice causes ataxia and generalized muscle twitching. Although cell-based assays did not demonstrate direct antigenicity, our findings point to Shaw-related subfamily of the potassium voltage-gated channels complexed proteins as hypothetical antigenic targets.
Keywords:
Immunoproteomics; Opsoclonus-myoclonus; Potassium channels; Synaptosomes.
Copyright © 2016 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Brain Neoplasms* / complications
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Brain Neoplasms* / immunology
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Brain Neoplasms* / pathology
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Cell Adhesion Molecules, Neuronal / metabolism
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Cell Line, Tumor
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Central Nervous System / metabolism*
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Central Nervous System / ultrastructure
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Child
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Databases, Factual / statistics & numerical data
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Encephalitis / complications
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Encephalitis / immunology
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Female
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HEK293 Cells
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Humans
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Male
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Membrane Proteins / metabolism
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Nerve Tissue Proteins / metabolism
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Nerve Tissue Proteins / ultrastructure
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Neuroblastoma* / complications
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Neuroblastoma* / immunology
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Neuroblastoma* / pathology
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Nuclear Proteins / metabolism
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Nuclear Proteins / ultrastructure
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Opsoclonus-Myoclonus Syndrome* / complications
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Opsoclonus-Myoclonus Syndrome* / immunology
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Opsoclonus-Myoclonus Syndrome* / pathology
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Rats
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Rats, Wistar
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Shaw Potassium Channels / immunology
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Shaw Potassium Channels / metabolism
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Shaw Potassium Channels / ultrastructure
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Synaptosomes / metabolism
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Synaptosomes / ultrastructure
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Thymoma / complications
Substances
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Cell Adhesion Molecules, Neuronal
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IgLON5 protein, human
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Membrane Proteins
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Nerve Tissue Proteins
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Nuclear Proteins
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Shaw Potassium Channels